维生素E对红细胞保存液保存效果改善的研究
Effect of Vitamin E on Human Erythrocyte Preservation Solutions
李晶哲 1刘剑 1李晓眠1
作者信息
- 1. 300070,天津,中国天津医科大学微生物实验室
- 折叠
摘要
目的 探索生物试验用红细胞体外保存时间延长的方法.方法 将健康成人的新鲜血液保存在已成熟应用的血液保存液CPDA和Alserve's中,并在两种保存液中均添加维生素E.在56天实验期间,每7天检测一次红细胞SOD、红细胞ATP、游离血红蛋白.结果 添加了维生素E10 mg/ml的红细胞保存液CPDA和Alserve's对红细胞的保护作用增强.在4"C保存条件下,准备应用于生物试验的红细胞的保存期限由28天提高到42天(CPDA)和由7天提高到21天(Alserve's).在最初7天保存期内两种保存液的效果近似.结论维生素E可以明显增强已成熟应用的血液保存液CPDA和Alserve's对红细胞的保护作用.珍贵血液样本在短期内使用建议选用配方简单的Alserve's+维生素E保存液.珍贵血液样本长期保存建议选用配方全面均衡的CPDA+维生素E保存液.
Abstract
Objective To explore a protocol for prolonging the in vitro preservation of red blood cells (RBCs)for biological tests.Methods The fresh blood of healthy adult was preservated in the well-established blood eell preservation solutions(CPDA and the Alserver's solution).After the preservation solutions were added by Vitamin E as an additive,the RBC-SOD,RBC-ATP,free hemoglobin(Free Hb)were examined every 7 days in 56-day experimental period.Results The average lifespan of RBCs had risen from 28 days to 42 days in CPDA+Vitamin E and from 7 days to 21 days in Alserve'S solution+Vitamin E respectively at CC.However,the preservation effects of two kinds of solutions are the same in initial 7 days.Conclusion Vitamin E can significantly enhance the protection effects of well-established erythroeyte prescrvation solutions.It is recommended that valuable RBCs sample can be preserved for short period of time in the simpled-component Alserve's with the additive Vitamin E.and be long-term saved with the balanced CPDA and Vitamin E.
关键词
红细胞保存/CPDA保存液/Alserve's保存液/维生素EKey words
red cell preservation/CPDA/Alserve'S/Vitamin EEffect of Vitamin E on Human Erythrocyte Preservation Solutions,LIU Jian,LJ Xiao-mian引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据