Toxicity of maternal zebrafish exposure to bisphenol A and its analogues on the development of offspring embryos
Objective To investigate the influence of continuous exposure of maternal zebrafish to bisphenol A(BPA)and its an-alogues on the development,behavioral characteristics,and circadian clock gene expression levels of offspring zebrafish.Methods A total of 70 female zebrafish,aged 4 months,were randomly divided into seven groups including control group,exposure groups of BPA,BPS,and BPF(0.1 and 10.0 μg/L,respectively),and after continuous exposure for 28 days,the eggs were collected to analyze and calculate the mortality,hatching,and malformation rates of offspring zebrafish.The eye size,eye distance,body length,heart rate,and relative blood flow of 72 hpf(hour post fertilization)juveniles and the level of reactive oxygen species in 96 hpf juveniles were pho-tographed and measured by stereomicroscope;Zebrabox behavior tracking analyzer was used to record the behavior activities of juvenile zebrafish;the expression levels of circadian clock genes was analyzed by qPCR.Results After 28 days of exposure to BPA,BPS,and BPF,compared with the control group,the mortality of all exposed groups was increased(P<0.05),the hatching rate,heart rate,and relative blood flow were decreased(P<0.05),the amplitude of spontaneous movement was decreased(P<0.05),and the content of reactive oxygen species and the expression levels of circadian clock genes were disturbed.Conclusion Maternal zebrafish exposure to BPA and its analogues may lead to abnormal development of offspring zebrafish through the changes in circadian clock genes.
bisphenol A(BPA)bisphenol A analogueszebrafishcircadian clockdevelopmental toxicity
万方数据
维普
