Objective:Discuss whether shionin can reverse the changes of hemodynamic and vascular remodeling in animal models of PAH by regulating interleukin-6 and downstream signal pathways.Methods:Twenty-four SD rats were randomly divided into CON group,MCT-PAH group and MCT-PAH+SH group.Four weeks later,pulmonary artery hemodynamics,indexes related to right heart function and right ventricular hypertrophy index were detected.Morphological changes of pulmonary artery were identified by HE staining.IL-6 was detected by immunofluorescence.Western blot and immunohistochemistry techniques were performed to detect the expression of IL-6,IL-21 in rats'lung tissues and the expression and localization of CD163 in lung tissues.Results:Compared with CON group,pulmonary artery blood flow acceleration time(PAAT)was shortened,the inner diameter of the right ventricle(RVID)was widened,the tricuspid annular plane systolic excursion(TAPSE)was decreased,right ventricular hypertrophy index was increased,right ventricular systolic pressure(RVSP)was increased,pulmonary artery vascular wall was significantly thickened in MCT-PAH group,and the vascular remodeling was marked by middle membrane hypertrophy.These results indicated that the animal model of pulmonary artery hypertension was successfully established.After the intervention with shikonin,the above indexes had different changes.Immunofluorescence results showed that the expression of IL-6 in pulmonary arterioles of MCT-PAH group was higher than that of CON group,and the expression of IL-6 in pulmonary arterioles of shikonin group was lower than that of MCT-PAH group.The results of western blot showed that the expression of IL-6creased in MCT-PAH group.Shikonin treatment reduced expression of IL-6,IL-21 in PAH rat's lung.The expression of CD163 in pulmonary blood vessels were significantly increased in MCT-PAH group rats,but reduced after shikonin treatment.Conclusions:Shikonin treatment alleviated PAH via improving hemodynamics of right ventricle and pulmonary vascular remodeling in experimental PAH.The effect may be relative to inhibition of IL-6 and its downstream signal pathway activation.
维普
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