Objective:To explore the effect of Mn3O4 nanoenzyme treatment on HIF-1α/BNIP3 signaling pathway in hypoxia/reoxygenation(H/R)cardiomyocytes.Methods:After establishing a H/R injury model using H9C2 mice,cardiomyocytes were randomly divided into five groups:control group,model group,Mn3O4 NP group,2-ME2 group and Mn3O4 NP+2-ME2 group.In each group,CCK-8 technique was used to determine myocardial cell viability,and Western blot and qRT-PCR were used to detect the protein and mRNA expression levels of HIF-1α,BNIP3,Beclin-1,P62,respectively.MDA and LDH levels were assessed by biochemical methods.Results:Compared with the control group,myocardial cell viability was decreased,LDH and MDA levels were increased,HIF-1α,BNIP3,Beclin-1 protein and mRNA expression levels were increased,and P62 expression was reduced in the model group(P＜0.05).Compared with the model group,myocardial cell viability was increased,LDH and MDA levels were decreased,HIF-1α,BNIP3 and Beclin-1 expression levels were increased,and P62 expression was decreased in the Mn3O4 NP group(P＜0.05).Opposite changes occurred in the 2-ME2 group(P＜0.05).Compared with the Mn3O4 NP+2-ME2 group,myocardial cell viability was increased,LDH and MDA levels were decreased,HIF-1α,BNIP3 and Beclin-1 expression levels were increased,and P62 expression was reduced in the Mn3O4 NP group(P＜0.05).Opposite changes were detected in the 2-ME2 group(P＜0.05).Conclusion:Mn3O4 NP may promote autophagy of H9C2 cardiomyocytes by activating HIF-1α/BNIP3 signaling pathway,thereby alleviating H/R-induced myocardial injury.
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