广西壮、苗族和越南京族人线粒体DNA 9 bp缺失频率的分析
Analysis of the Frequencies of mtDNA 9bp Deletion in Zhuang and Miao Populations from Guangxi and Kinh Population from Vietnam
方玲 1胡启平 1潘尚领 1马军 1季米娜 1窦霄云 1陈晶 1陈文诚 1袁志刚1
作者信息
- 1. 530021,南宁,广西医科大学细胞生物学与遗传学教研室
- 折叠
摘要
目的 探讨广西壮、苗族和越南京族人的mtDNA V区的遗传多态性.方法 PCR-PAGE扩增和检测COⅡ与tRNALys 基因之间的片段,随机选择部分PCR扩增产物进行测序予以确认.结果 在广西壮、苗族和越南京族人样本中只发现标准型和短型两种类型的扩增产物.广西壮、苗族和越南京族人的9 bp缺失频率分别为20.83%、33.01%和33.31%.结论 来自不同地区种族和人群的9 bp缺失频率有所不同.研究结果支持中国南方人群与越南人在遗传上有较为密切的关系.
Abstract
Objective To explore the polymophism of mitochondrial DNA region V existed in Zhuang and Miao populations from Guangxi province of China and Kinh From Vietnam. Methods The fragments between the CO Ⅱ and tRNAlyz genes were amplified by PCR and some of the PCR products were sequenced to detect the types of 9 bp deletion. Results Only standard pattern and short pattern were found in the samples studied. The frequencies of the 9 bp deletions in Zhuang and Miao populations from Guangxi and Kinh from Vietnam were 20. 83%, 33.01% and 31. 31%, respectively. Conclustion The deletion occus in various frequencies in different populations. The data suggest close genetic relationship between the populations from southern part of China and Vietnam based on the frequencies of 9 bp deletions in V region of mtDNA.
关键词
壮族/苗族/越南京族/线粒体DNA/9/bp缺失Key words
Zhuang/Miao/Kinh/Mitochontrial DNA/9 bp deletion引用本文复制引用
出版年
2007
NETL
NSTL
维普
万方数据