Detection of the stability of DPS nano capsule via circular dichroism
Objective To study the effects of single amino acid mutation on the stability of DNA-binding proteins from starved cells(DPS), and to explore the relationship between the amino acid sequence and the structure of whole nano capsule.Methods The amino acids which might affect the conformation were mutated artificially.Circular Dichroism(CD) was applied to collect the parameters of the molecule structure.Results Single amino acid mutation could cause huge changes to the percentage of α helix of the DPS capsule.Conclusions The amino acids are important to keep DPS molecule intact.The mutations could introduce changes to the structure of the molecule and thus change its feature and function, which may help us better understand the relationship between amino acid sequence and molecule structure and function.
DNA-binding proteins from starved cellscircular dichroismmutationprotein purification
NETL
NSTL
万方数据
维普
