抵抗素样分子α对小鼠主动脉内皮细胞形成新生血管能力的影响及机制探讨
Effects and Mechanism of Resistin-like Molecules α on Endothelial Cell Angiogenesis in Mouse Aorta
段淑香 1孟海燕 1孙萍 1张宁 1牛洪梅 1赵中松 1李浩元 1马永晖1
作者信息
- 1. 山东省立第三医院心肺康复科,济南 250031
- 折叠
摘要
目的 探讨抵抗素样分子α(又称FIZZ1)对小鼠主动脉内皮细胞形成新生血管能力的影响及机制.方法 分别使用10 nmol/L、20 nmol/L和40 nmol/L的FIZZ1刺激小鼠主动脉内皮细胞,使用内皮细胞成管实验和内皮细胞迁移实验体外验证FIZZ1在内皮细胞迁移及血管形成中的作用;采用高通量测序检测FIZZ1对小鼠主动脉内皮细胞信号通路的影响,筛选出表达有显著差异的信号通路.结果 在不同浓度的FIZZ1刺激下,小鼠主动脉内皮细胞血管形成的面积逐步增加,40 nmol/L的FIZZ1促进血管生成的作用最强.随着FIZZ1浓度的增加,内皮细胞迁移数量增多,迁移能力逐步增强.高通量基因测序检测中发现共有1 253个基因出现了差异性表达,包括427个上调基因和826个下调基因,其机制可能与COL1A1、FLT4、ITGA3等基因表达显著性差异有关.结论 FIZZ1能在体外使小鼠主动脉内皮细胞形成新生血管,其机制可能与COL1A1、FLT4、ITGA3等基因表达显著性差异有关.
Abstract
Objective To explore the effects and mechanism of resistin-like molecules α(also known as FIZZ1)on the ability of mouse aortic endothelial cells to form new blood vessels.Methods Mouse was stimulated by FIZZ1 at 10 nmol/L,20 nmol/L and 40 nmol/L.The role of FIZZ1 in endothelial cell migration and blood vessel formation was verified by using endothelial cell tube formation experiments and endothelial cell migration experiments in vitro;The effect of FIZZ1 on signaling in mouse aortic en-dothelial cells was detected by high-throughput sequencing,and pathways with significant differential expression signaling were se-lected.Results Under different concentrations of FIZZ1,the area of blood vessel formation in mouse aortic endothelial cells gradual-ly increased,and 40 nmol/L FIZZ1/RELMα had the strongest effect in promoting angiogenesis.With the increasing concentration of FIZZ1,the number of endothelial cells migrated increased,and the migration capacity gradually increased.A total of 1 253 genes were found to be differentially expressed,including 427 up-regulated genes and 826 down-regulated genes.The mechanism may be related to the significant expression difference of COL1A1,FLT 4 and ITGA 3.Conclusion FIZZ1 can induce neovascularization of mouse aortic endothelial cells in vitro,and its mechanism may be related to significant differences in gene expression such as COL1A1,FLT4,and ITGA3.
关键词
抵抗素样分子α/血管新生/内皮细胞/主动脉/小鼠Key words
Resistin-like molecules α/Angiogenesis/Endothelial cells/Aorta/Mice引用本文复制引用
基金项目
山东省医药卫生科技发展计划(202103010999)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据