Objective To explore circular RNAs(circRNAs)that regulate ferroptosis in cervical cancer,in the develop-ment of new therapeutic targets.Methods The differentially expressed circRNAs(DEcircRNAs),differentially expressed microR-NAs(DEmiRNAs)and differentially expressed mRNAs(DEmRNAs)between cervical cancer and control groups were analyzed based on GSE102686,TCGA,GSE9750.circRNAs and mRNAs with targeted regulatory relationships with DEmiRNAs were predicted by the Starbase database and compared with differential analysis results.Enrichment analysis of mRNAs was performed to identify mRNAs associated with cell death(cell cycle,apoptosis,and iron death)and to construct relevant competitive endogenous RNAs(ceRNAs)regulatory networks.Univariate Cox regression analysis was performed to identify miRNAs and mRNAs that significantly affect patient outcomes.Cervical cancer tissues and distant control cervical tissue samples from 12 patients with cervical cancer were collected to de-tect gene expression using quantitative reverse transcription polymerase chain reaction(qRT-PCR)and Western blot,and targeted binding of genes was detected using a dual-luciferase reporter.Results A total of 179 DEcircRNAs,93 DEmiRNAs,and 739 DEmRNAs were identified between cervical cancer and controls,and 160 DEcircRNAs were found,and 565 DEmRNAs were targeted to DEmiRNAs.The ceRNAs regulatory network of circ_0001461/miR-145-5p/transferrin receptor(TFRC)was finally identified to be associated with ferroptosis.circ_0001461 and TFRC were significantly upregulated and miR-145-5p was downregulated in cervical cancer tissues(P＜0.05).The protein expression of TFRC in cervical cancer tissues was significantly upregulated(P＜0.05).In addition,miR-145-5p had target binding ability to both circ_0001461 and TFRC(P＜0.05).Conclusion The ceRNAs network of circ_0001461/miR-145-5p/TFRC regulates ferroptosis and is associated with the prognosis of patients with cer-vical cancer,which may provide novel therapeutic approaches for patients with cervical cancer.
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