老年急性缺血性脑卒中患者血清miR-340-5p及STING水平与病情及预后的关系
The Relationship between Seru miR-340-5p,STING Levels and Disease Condition and Prognosis in Elderly Patients with Acute Ischemic Stroke
马艳 1胡芳 1吕学海 1刘运平 1王晓莹 1柴佳 1郭一诺 1朱晓帅1
作者信息
摘要
目的 探究老年急性缺血性脑卒中患者血清 miR-340-5p 及干扰素基因刺激蛋白(STING)水平与病情及预后的关系.方法 选择邯郸市中心医院 2020 年 5 月—2022 年 5 月收治的老年急性缺血性脑卒中患者 113 例作为研究组,另选取同期正常健康者 113 例作为对照组.根据患者入院当天的美国国立卫生研究院卒中量表(NIHSS)评分评估神经缺损程度(<6 分为轻度,6～<14 分为中度,≥14 分为重度),根据治疗 3 个月后改良 Rankin 量表(mRs)评分评估预后[预后良好(0～2 分)、预后不良(≥3 分)].采用实时荧光定量 PCR 法检测血清 miR-340-5p、STING 表达水平,多因素 logistic 回归分析预后的影响因素.ROC 曲线分析血清 miR-340-5p、STING 对预后的预测价值.结果 研究组血清miR-340-5p 表达水平低于对照组(P<0.05),血清 STING 表达水平高于对照组(P<0.05).神经缺损程度轻度 37 例、中度 43 例、重度 33 例,血清 miR-340-5p 在不同神经缺损程度患者中的表达水平为重度者<中度者<轻度者(P<0.05),而血清 STING 在不同神经缺损程度患者中的表达水平为重度者>中度者>轻度者(P<0.05).预后良好 74 例,预后不良 39 例,预后不良组血清 STING 水平、入院时 NIHSS 评分均高于预后良好组(P<0.05),而血清 miR-340-5p 水平低于预后良好组(P<0.05).多因素 logistic 回归分析结果显示:血清 miR-340-5p 为预后的保护因素(P<0.05),血清 STING 及 NIHSS 评分为其危险因素(P<0.05);ROC 曲线显示,血清 miR-340-5p、STING 联合预测预后的曲线下面积大于各指标单独预测(P<0.05).结论 血清 miR-340-5p 及 STING 表达水平均能反映老年急性缺血性脑卒中患者的病情,在预后预测方面具有一定的临床价值.
Abstract
Objective To investigate the relationship between serum miR-340-5p and stimulator of interferon gene(STING)levels in elderly patients with acute ischemic stroke and their condition and prognosis.Methods A total of 113 elderly pa-tients with acute ischemic stroke admitted to Handan Central Hospital from May 2020 to May 2022 were selected as the study group,and 113 normal healthy patients during the same period were selected as the control group,according to the neurological impairment was assessed according to the National Institutes of Health Stroke Scale(NIHSS)score on the day of admission(<6 scores as mild,6-<14 scores as moderate,≥14 scores as severe),and prognosis was assessed according to the modified Rankin scale(mRs)score after3 months of treatment[good prognosis(0-2 scores)and poor prognosis(≥3 scores)].The expression levels of serum miR-340-5p and STING were detected by real-time fluorescence quantitative PCR.The influencing factors of prognosis were analyzed by multivariate logistic regression.ROC curve was used to analyze the predictive value of serum miR-340-5p and STING for prognosis.Results The expression level of serum miR-340-5p in study group was lower than that in control group(P<0.05),and the expres-sion level of STING in serum was higher than that in control group(P<0.05).There were 37 cases with mild nerve defects,43 ca-ses with moderate nerve defects,and 33 cases with severe nerve defects.The expression level of serum miR-340-5p in patients with dif-ferent nerve defects was severe<moderate<mild(P<0.05),while the expression level of serum STING in patients with different nerve defects was severe>moderate>mild(P<0.05).There were 74 patients with good prognosis and 39 patients with poor prognosis.Serum STING level and NIHSS score at admission in the poor prognosis group were higher than those in the good prognosis group(P<0.05),while serum miR-340-5p level was lower than those in the good prognosis group(P<0.05).Multivariate lo-gistic regression analysis showed that serum miR-340-5p was the protective factor for prognosis(P<0.05),and serum STING and NIHSS scores were the risk factors(P<0.05).ROC curve showed that the combined prognostic area of serum miR-340-5p and STING was greater than that predicted by each index alone(P<0.05).Conclusion The expression levels of serum miR-340-5p and STING can both reflect the condition of elderly patients with acute ischemic stroke,and have certain clinical value in prognostic prediction.
关键词
miR-340-5p/干扰素基因刺激蛋白/急性缺血性脑卒中/预后Key words
miR-340-5p/Stimulator of interferon gene/Acute ischemic stroke/Prognosis引用本文复制引用
基金项目
邯郸市科学技术研究与发展计划项目(22422083027ZC)
出版年
2024
国家科技期刊平台
NSTL
万方数据