Compound Screening of High-yield Strain of Streptomyces avermitilis
Objective The purpose of this study is to screen high-yield strains of Streptomyces avermitilis.Methods In this study,two breeding methods,ARTP mutation and aveR gene overexpression,were used to screen Streptomyces avermitilis.Results The RW-51 mutant strain used in this investigation had a titer of 7 268 U/mL,which was 8.9％greater than the original strain after ARTP mutagenesis screening.When the aveR gene was constructed and overexpressed,a high-yield strain RW-997 was screened with a titer of 7 649 U/mL,which was 14.6％greater than that of the original strain and 7.7％higher than that of the mutant strain RW-5.Conclusion One high-yielding strain RW-997 was selected for large-scale production.
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