十二碳二元酸菌种发酵工艺优化
Optimization of Fermentation Process of Dodecarbon Acid bacteria strains
任勇 1张会萍 1张萍 1牛春1
作者信息
- 1. 宁夏泰瑞制药股份有限公司,银川 750100
- 折叠
摘要
目的 以热带假丝酵母菌为试验菌株,通过发酵工艺优化,提高菌种发酵产酸量.方法 对发酵培养基中碳源和氮源等成分进行优化,同时研究了种龄、发酵培养温度、摇瓶转速、发酵接种量、发酵瓶装量和发酵pH值对发酵产酸量的影响.结果 发酵培养最适碳源为2 g/L蔗糖,最适氮源为3 g/L酵母浸膏,菌体发酵在28 ℃,200 r/min,25 mL/250 mL装量,以15％接种量条件下培养,发酵pH值调至7.0～7.5时,十二碳二元酸平均产量可达到130 g/L.结论 对发酵培养基碳氮源成分进行优化、发酵工艺进行优化,发酵产酸量提高至130g/L.同时发酵培养基中添加氯化钠和硫酸镁后,均不利于菌种产酸.
Abstract
Objective To improve the acid production of Candida tropicalis by optimizing the fermentation process.Methods The effects of seed age,fermentation culture temperature,shake flask rotation speed,inoculation volume,filling volume,and sources for the 2 g/L of sucrose,the ideal nitrogen source for 3 g/L extract yeast,bacteria fermentation at 28 ℃,and fermentation pH value on acid production were also studied.The carbon source,nitrogen source,and other components in the fermentation medium were optimized.Results Fermentation to develop the optimal carbon 200 r/min,25 mL/250 mL bottle,with a 15％seed volume,under the condition of fermentation pH value to 7.0～7.5,the average yield of dodecarbonate can reach 130 g/L.Conclusion The carbon and nitrogen supply components of the fermentation medium were improved,along with the fermentation process,resulting in an increase in the fermentation acid production to 130 g/L.At the same time,The addition of sodium chloride and magnesium sulfate in the fermentation medium is not conducive to the production of acid by the strains.
关键词
热带假丝酵母菌/碳源/氮源/十二碳二元酸/优化/产酸量Key words
Candida tropicalis/carbon source/nitrogen source/dodecarbonate/optimize/acid production of the strain引用本文复制引用
出版年
2024
NETL
NSTL
万方数据