摘要
为了研究酸枣仁这种传统中药的抗氧化活性，采用多项抗氧化活性指标进行评价，通过活性追踪，酸枣仁提取物依次经过乙酸乙酯萃取，聚酰胺树脂分离和 C18反相快速制备色谱分离，顺次得到抗氧化活性最好的萃取物、馏分B和黄酮组分6''-阿魏酰斯皮诺素，同时还分离得到另外两个组分斯皮诺素和酸李碱。在进一步比较了三种组分酸李碱、斯皮诺素与6''-阿魏酰斯皮诺素抗氧化活性的基础上，将三种组分按照不同配比混合后进行了抗氧化活性的研究，证明这三种物质在清除DPPH自由基的反应中呈现拮抗作用，在总抗氧化能力的反应中为拮抗作用，而在与金属离子螯合能力的反应中为协同作用。以上结果证明酸枣仁抗氧化活性的有效部位是黄酮，抗氧化活性最强的组分为6''-阿魏酰斯皮诺素，但是酸枣仁黄酮组分之间的抗氧化活性存在一定程度的拮抗作用。
Abstract
In order to study the antioxidant activity of Zizyphi Spinosi Semen (ZSS) which is known as a traditional Chinese herb for treatment of insomnia and anxiety, extracts of ZSS was extracted by ethyl acetate, separated by polyamide resin and isolated by C18 reverse-phase medium, orderly. The antioxidant activity of products obtained from each step was measured. The extracts of ethyl acetate, fraction B and 6' '-feruloylspinosin have the best antioxidant activity. At the same time, other two effective ingredients zizyphusine and spinosin were also separated. The antioxidant activity of the three effective ingredients was measured. The antioxidant activity was also studied by mixing zizyphusine, spinosin and 6' '-feruloylspinosin with different proportions. The results prove that the three ingredients have antagonistic effect on both the removal of free radical DPPH and total antioxidant capacity. But they have synergistic effect on chelating with metal ions. The results show that the flavone is the effective part of ZSS for the antioxidant activity, 6' '-feruloylspinosin is the ingredient of flavone in ZSS with the best antioxidant activity. However antagonistic effect on antioxidant activity of ZSS exists among the ingredients of flavone in ZSS .
基金项目
北京市优秀人才培养基金资助项目(2008ID0502100229)
北京市重点建设学科资助项目(PXM2009_014207_078172)
北京农学院青年基金重点项目(2066016013)
北京农学院优势科研团队支持项目()
国家科技期刊平台
NSTL
万方数据