半疏水晶胶微球的悬浮特性及固载乳杆菌合成有机酸
Suspension characteristics of semi-hydrophobic cryogel beads and their application in Lactobacillus paracasei cell immobilization for organic acid production
侯东杰 1楼小玲 2蔡礼年 2陈治宏 2张玮 1陈冰冰 1贠军贤2
作者信息
- 1. 浙江工业大学 机械工程学院,浙江 杭州 310023
- 2. 浙江工业大学 绿色化学合成技术国家重点实验室培育基地,浙江 杭州 310014
- 折叠
摘要
针对有机酸生物合成过程中底物和产物抑制、细胞密度低和耐受性弱等问题,采用"结晶致孔-低温聚合"方法制备聚甲基丙烯酸羟乙酯-甲基丙烯酸丁酯半疏水晶胶微球,通过高速成像和数值模拟研究 10 L搅拌式生物反应器(STR)内的悬浮行为,通过固载乳杆菌分析半疏水晶胶微球在乳酸和苯乳酸生物合成中的作用.结果表明,数值模拟得到晶胶微球的悬浮特性与高速成像实验结果基本一致,体积分数为 3%的晶胶微球均匀悬浮所需最低搅拌转速为 55 r·min-1;在晶胶固载乳杆菌发酵联产乳酸和苯乳酸过程中,乳杆菌生物量达 10.5 g·L-1,相比于无载体发酵提高了 84.2%;在第 1 次补料期间,乳酸的发酵周期大幅缩短至 21 h,比无载体发酵周期缩短了 50%.
Abstract
In order to solve the problems of substrate and product inhibition,low cell density and organic acid tolerance,poly(hydroxyethyl methacrylate-butyl methacrylate)semi-hydrophobic cryogel beads were prepared by"crystallization pore-forming and low temperature polymerization",and their suspension behaviors in a 10 L stirred bioreactor were investigated by high-speed imaging and numerical simulation.Lactic acid and phenyllactic acid biosynthesis processes were studied using these cryogel bead immobilized Lactobacillus.The results show that the suspension characteristics of the cryogel beads obtained by numerical simulation are consistent with those of high-speed imaging,and the minimal stirring rate required for uniform suspension of cryogel beads(volume fraction is 3%)is 55 r·min-1.The Lactobacillus biomass reached 10.5 g·L-1 in the co-production process of lactic acid and phenyllactic acid,which was increased by 84.2%compared with Lactobacillus fermentation without immobilization.During the first feeding period,the fermentation period of lactic acid was greatly shortened to 21 h,which was 50%shorter than that without immobilization.
关键词
半疏水晶胶微球/搅拌式生物反应器/悬浮行为/乳杆菌/发酵/数值模拟Key words
semi-hydrophobic cryogel bead/stirred tank bioreactor/suspension behavior/Lactobacillus/fermentation/numerical simulation引用本文复制引用
基金项目
浙江省自然科学基金重大项目(LD21B060001)
国家自然科学基金(22078296)
国家自然科学基金(21576240)
出版年
2024
国家科技期刊平台
NSTL
万方数据