Chromatographic adsorption characteristics of phenyllactic acid by cryogel beads embedded with nanocellulose
Cryogel beads embedded with nanocellulose were prepared using a multi-micro-tube reactor,which were grafted with N,N-dimethylaminoethyl methacrylamide and used as the chromatographic adsorbents in the bioproduction of phenyllactic acid.The results show that these cryogel beads have particle sizes of 4 to 6 mm,over 70%effective porosity and 87%to 91%absolute porosity.The adsorption properties of cryogel beads embedded with different contents of cellulose(mass fractions of 1%,3%and 5%,respectively)packed in columns were investigated for bovine serum albumin adsorption,and the adsorption capacities were 1.72,2.84 and 4.49 mg·mL-1,respectively.The phenyllactic acid adsorption conditions were optimized experimentally.The results show that the adsorption capacity decreases with the increase of sample flow rate and the maximum adsorption capacity is 41.32 mg·mL-1 at the sample solution pH of 6.Five runs of chromatographic adsorption and desorption of phenyllactic acid were repeated in the packed column and the adsorption capacity were between 24.17 and 26.31 mg·mL-1,which indicates that the cryogel beads have potential for phenyllactic acid separation in bioproduction.
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