Multi-wavelength lasers coupling system used in fluorescent microscopy
The rapid advancement of fluorescence microscopy technology has increased the need for better excitation light sources,particularly for multi-wavelength lasers coupled in single mode fiber.This article explores the technology of coupling multi-wavelength lasers into single mode fibers within the spectral range of 400-680 nm.To enhance the coupling efficiency of different wavelengths,a triplet lens is de-signed to effectively reduce chromatic aberration.Moreover,a fiber coupling component structure and a quick and easy coupling adjustment method was developed to address the challenges of fiber coupling align-ment.Fiber coupling experiments involving four commonly used laser wavelengths in fluorescence microsco-py(405,488,561,638 nm)have demonstrated a coupling efficiency of over 65%for all four wavelengths.These results validate the effectiveness of the multi-wavelength lasers coupler designed in this study.Further-more,due to its simple adjustment and low cost,the coupler holds significant commercial value and estab-lishes a solid foundation for the localization of multi-wavelength lasers coupled in single mode fiber.
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