Daul-optical path observation system for tracking free-moving C.elegans
To overcome challenges related to a limited field of view and susceptibility to environmental fac-tors impacting data accuracy,we have developed a free-moving Caenorhabditis elegans(C.elegans)dual-optical observation system.This system integrates both a microscopy imaging module and a large-field-of-view imaging module,allowing for the simultaneous capture of C.elegans microscope images and Petri dish images.By incorporating a switchable fluorescence module,the system meets the requirements for capturing C.elegans fluorescence images.Additionally,the system includes an XYZ three-axis platform to prevent vibration-triggered escape responses from C.elegans.We utilized this system to conduct imaging experiments on USAF1951 resolution plates and various C.elegans strains.The experimental results dem-onstrated exceptional microscopy imaging resolution of less than 2.19 mm,vividly displaying C.elegans'organs,tissues,and cell boundaries in bright-field microscopy images,along with clear imaging of various fluorescent protein distributions.The large-field-of-view module,featuring uniform illumination and a mor-phological algorithm,enables the collection of behavior and motion data from the C.elegans population.In summary,this system fulfills various C.elegans tracking requirements,exhibits significant stability and da-ta accuracy,enhances the flexibility of C.elegans experiments,and lays a foundation for integrating C.ele-gans tracking data across different scales.
microscopy imagingdaul-optical path imagingC.elegansC.elegans observing system
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