Continuous monitoring chip of blood pH and PCO2 based on electrochemical sensing
For continuous assessment of the acid-base balance in the blood of hemodialysis patients and re-ducing mortality risk,this work proposes a chip for continuous monitoring of pH and PCO2 in blood,utiliz-ing electrochemical sensing and microfluidic technology.The chip combines pH and PCO2 detection via a pH electrode and a carbonate ion-selective electrode,sharing a common reference electrode.The microflu-idic test chamber ensures continuous blood flow and accurate detection by preventing air interference.The chip's pH detection performance was tested using Tris-HCl solutions ranging from pH 6.00 to 9.00,show-ing a detection sensitivity of 61.2 mV/pH with a standard deviation under 0.01(n=10)for continuous de-tection of the same sample.The reproducibility test showed average relative errors of 0.46%(n=6),ensur-ing accurate detection of sample solutions within the pH 6.00-9.00 range.The sensor was tested in both blood gas control solutions and blood samples.In blood gas control solutions,the absolute errors for pH and PCO2 detection were under 0.04 and 2.30 mmHg,with average relative errors of 0.23%and 1.94%,respectively.In blood samples,the absolute errors for pH and PCO2 detection were under 0.05 and 3.50 mmHg,with average relative errors of 0.36%and 2.60%,respectively.Compared to commercial blood gas analyzers,the chip's small size allows it to connect to the hemodialysis pipeline,enabling continuous online detection of pH and PCO2 in blood.
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