首页|苦瓜抗白粉病SCAR分子标记的开发

苦瓜抗白粉病SCAR分子标记的开发

Development of SCAR from SRAP molecular markers closely linked to powdery mildew-resistant genes in bitter melon (Momordica charantia L.)

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
  • 维普
[目的]获得与苦瓜抗白粉病基因紧密连锁的分子标记,为加快苦瓜抗白粉病新品种的选育奠定基础.[方法]以高抗白粉病野生苦瓜MC18为父本、高感白粉病苦瓜栽培种MC1-2为母本创建F2代分离群体;经单株抗病性鉴定后,以BSA法构建F2代单株的高抗和高感白粉病苦瓜DNA近等基因池;利用SRAP技术筛选多态扩增片段,对仅在抗白粉病近等基因池和父本中出现的差异片段进行同收、测序、比对和转化成SCAR标记,并利用已知抗病性的单株DNA分析标记与苦瓜抗白粉病的相关性.[结果]从1188对SRAP引物组合中筛选到稳定阳性差异条带的引物组合5对,其中ME20EM5引物对扩增的差异条带长度为332bp,与葡萄抗体蛋白基因(抗性基因)的DNA序列有较高相似性,并将其成功转化成与苦瓜白粉病抗性相关、大小为320 bp的SCAR标记.[结论]开发的SCAR-ME20EM5分子标记可用于苦瓜抗白粉病分子标记辅助选择.
[Objective]Molecular markers closely linked to powdery mildew resistant genes in bitter melon (Momordica charantia L.) were obtained to provide basis on accelerating the development of new powdery mildew resistant cultivars.[Method]The plants from F2 generations were created through the hybridization of highly powdery mildew-sensitive and cultivated bitter melon MC1-2 as female parent with strongly powdery mildew-resistant and wild bitter melon MC18 as male parent.After identification powdery mildew resistance abilities with their parents,the near-isogenic DNA pools from F2 population plants were classified on the basis of bulked segregation analysis (BSA) method.Polymorphic amplified fragments were screened from near-isogenic DNA pools by sequence-related amplified polymorphism (SRAP) technique.The differential fragments only belonging to powdery mildew-resistant near-isogenic pool and paternal plant were retrieved,sequenced,blasted,and changed into SCAR marker.The correlations of sequence characterized amplified region (SCAR) molecular marker with powdery mildew-resistant abilities were analyzed based on DNA of the known disease-resistant or-sensitive plants.[Result]Five pairs of SRAP primers in 1188 had stable and positive differential fragments,of which ME20EM5 primer combination not only got a differential fragment with 332 bp sharing comparatively high similarity with gene encoded grape antibody protein (disease-resistant gene),but also changed into 320 bp SCAR molecular marker significantly correlated with powdery mildew resistance.[Conclusion]SCAR molecular marker screened could be used to develop powdery mildew-resistant bitter melon using marker-assisted selection.

bitter melon (Momordica charantia L.)powdery mildewbulked segregation analysisSRAPSCAR

周生茂、尚小红、梁任繁、郭元元、文俊丽、黄皓、陈振东、班美玲、黄如葵

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广西农业科学院蔬菜研究所,南宁530007

广西作物遗传改良生物技术重点开放实验室,南宁530007

广西环境保护科学研究院,南宁530022

苦瓜 白粉病 集团分离分析法 SRAP SCAR

国家自然科学基金广西科学研究与技术开发计划项目广西自然科学基金广西农业科学院基本科研业务专项项目广西农业科学院公益性维持费项目

31240060桂科攻10100005/62012GXNSFAA053061桂农科2012YT05桂农科2012GW04

2013

10.3969/j:issn.2095-1191.2013.10.1595

南方农业学报
广西壮族自治区农业科学院

南方农业学报

CSTPCDCSCD北大核心
影响因子：0.83
ISSN：2095-1191
年,卷(期)：2013.44(10)
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