Establishment of a rapid propagation system in vitro for Citrus paradisi Macf.
[Objective]A stable and efficient in vitro propagation system for grapefruit was established in order to provide useful references for grapefruit gene transformation and large-scale expansion.[Method]The stems with auxiliary buds of aseptic seedlings from introduced Henderson Ruby,Hudson Foster,Perlist,Reed Marsh,and Thailand were used as explants to explore the effects of different concentrations of 6-BA,IBA and different genotypes on the induction and proliferation of adventitious bud,and rooting culture.[Result]The highest budding rate of grapefruit was observed in the medium with 0.25 mg/L and 0.50 mg/L 6-BA,which was 100.0％.In the range of 0.10-0.40 mg/L IBA,the adventitious buds were induced.In the medium MS+0.50 mg/L 6-BA+0.20 mg/L IBA,Perlist and Thailand had the highest budding rate,which was 96.67％ and 97.78％.The medium for multiplication of the regeneration buds of Hudson Foster and Reed Marsh was MS+0.25 mg/L 6-BA+0.20 mg/L IBA.The rooting medium of Hudson Foster,Perlist and Reed Marsh was 1/2MS+2.00 mg/L IBA.The medium for rooting of the other grapefruit varieties was 1/2 MS+1.00 mg/L IBA.The survival rate of regenerative plant was the highest in Thailand,which was 87.88％.The lowest was Henderson Ruby,which was only 48.48％.[Conclusion]The rapid in vitro regeneration system is established by means of culturing the stems with auxiliary buds from five varieties of grapefruit.
grapefruitstems auxiliary budadventitious rootin vitro culturerapid propagation system
国家科技期刊平台
NSTL
万方数据
维普
