Diversity analysis of endophytic bacterial separated from the leguminous plants nodules of Wenshan rocky desertification areas
[Objective]Diversity of endophytic bacterial separated from the leguminous plants nodules of Wenshan rocky areas was analyzed to provide theoretical references for the legume used in ecological restoration and reconstruction of rocky desertification area.[Method]Using YMA and NA medium,the endophytic bacteria of six legume nodules which were collected from Wenshan esertification region,were isolated and purified.On this basis,restriction enzyme digestion (removing redundant),ARDRA,16S rDNA sequence homology and biological phenotypic characteristics were analyzed.[Result]The results showed that 135 endophytic bacteria were identified as twenty four species,belonging to seven genera.Among them,49 strains belonged to Pseudomonas(6 kinds),17 strains belonged to Pantoea (3 kinds),25 strains belonged to Enterobacter(6 kinds),17 strains belonged to Bacillus(5 kinds),14 strains belonged to Paenibacillus (1 kind),10 strains belonged to Rhizobium (2 kinds) and 3 strains belonged to Agrobacterium(lkind).Phenotypic analysis showed that SWFU-W032 strain could grow in the 5％ salt medium;13 endophytic bacteria strains could grow in the pH 12.0 medium;SWFU-W024 and SWFU-W009 strains hydrolyzed starch;SWFU-W011,SWFU-W015 and SWFU-W020 strains synthesized antibiotic;except SWFU-W60,all strains had the ability to produce IAA as well as SWFU-W045,SWFU-W046 and SWFU-W070 strains had the ability to produce protease.[Conclusion]The endophytic bacteria of legume in Wenshan rocky desertification region are quite diversified.Some strains with higher resistance and strong tolerance can promote plant growth and these strains can be used for rocky desertification soil improvement and ecological restoration in rocky desertification areas.
国家科技期刊平台
NSTL
万方数据
维普
