[Objective]Effects of stem positions and growing areas on usability of tobacco stem were analyzed,in order to provide references for reasonably using tobacco stem resource and designing cigarette formula.[Method]The physical properties,sensory quality,harmful components in mainstream cigarette smoke and hazard index of tobacco cut stems were analyzed,these cut stems were from 3 positions(upper,middle and lower) and six growing areas(Chuxiong of Yunnan,Bijie of Guizhou,Chongqing,Chengzhou of Hunan,Nanping of Fujian,Pingdingshan of Henan).[Result]The results showed that,the order of physical quality was middle stem>upper stem>lower stem.The physical quality of cut stems from Chuxiong,Nanping and Bijie was better than those from Chongqing and Chenzhou.The order of sensory quality was upper stem>middle stem>lower stem,the sensory quality of cut stems from Chongqing was lower,and there was non-significant difference in sensory quality among other growing areas.The order of released tar content was upper stem>middle stem and lower stem,The released tar content of cut stems from Pingdingshan,Chengzhou and Chongqing was better than those from Nanping and Bijie.The order of released carbon monoxide(CO) content of cut stems from Chuxiong and Nanping was middle stem>upper stem>lower stem,but that of cut stems from other growing areas was upper stem>middle stem> lower stem,and released CO content of cut stems from Pingdingshan and Chuxiong was higher.The order of hazard index was upper stem>middle stem>lower stem,the hazard indexes of cut stems from Pingdingshan,Chuxiong and Chongqing were higher than those from other growing areas.[Conclusion]The stem position and growing area are the important factors affecting usability of tobacco stems.The tobacco stems should be selected reasonably according to quality index requirements of cigarette products in actual production.