Comparative analysis of SRAP and AFLP markers for genetic diversity of Blumea balsamifera D C.
[Objective]The genetic diversity of Blumea balsamifera D C.was estimated,in order to provide reference basis for its protection strategy.[Method]Based on AFLP and SRAP molecular markers,the genetic diversity of 35 B.balsamifera accessions was estimated.[Result]A total of 1360 AFLP polymorphism bands (polymorphic ratio of 99.48％)and 265 SRAP polymorphism bands(polymorphic ratio of 97.78％) were amplified using 8 AFLP primer combinations and 27 SRAP primer combinations.Polymorphism information content (PIC),effective multiplex ratio (EMR) and marker index(MI) of SRAP molecular marker were 0.4381,8.1000 and 4.3141,respectively.PIC,EMR and MI of AFLP molecular marker were 0.1773,198.0000 and 301.1348,respectively.Based on AFLP and SRAP polymorphic bands,the genetic similarity of 35 accessions ranged from 0.4450 to 0.9179,and the correlation coefficient(r) was 0.47.The UPGMA clustering results showed that,based on genetic similarity coefficient of SRAP molecular marker,35 accessions could be classified into 5 clusters,but based on genetic similarity coefficient of AFLP molecular marker,35 accessions could be classified into 4 clusters.[Conclusion]Both SRAP and AFLP molecular markers can used to estimate genetic diversity of B.balsamifera,but AFLP molecular marker is more suitable to estimate genetic diversity of B.balsamifera because it has more polymorphic sites and higher marker characteristic index.
Blumea balsamifera D C.genetic diversityAFLPSRAPcomparative analysis
国家科技期刊平台
NSTL
万方数据
维普
