Effects of four treatments on seed germination of Rosa chinensis
[Objective]The present study was conducted to analyze effects of different treatments on germination of Rosa chinensis seeds in order to provide a theoretical and technical support for raising seed germination rate and breeding efficiency of R.chinensis.[Method]Using R.chinensis seeds as material,the effects of cold stratification,different hormone concentrations,different substrates and aseptic seeding on seed germination were studied.[Result]Results showed that:after cold stratification treatnent,seed germination rate reached 42.22% and germination energy was 24.44%;100.0 mg/L GA3 and 100.0 mg/L NAA could significantly raise germination rate and germination energy of R.chinensis(P<0.05).After 100.0 mg/L GA3 treatment,seed germination rate was 55.56% and germination energy 42.22%;in 100.0 mg/L NAA treatment,seed germination rate reached 48.89% and germination energy 30.00%.In terms of R.chinensis planted in river sand,the germination rate was 42.22% and germination energy 24.44%,which were better than those in peat-perlite matrix (volume ratio 2∶1) and garden soil.Adding GA3 in MS media was beneficial for seed germination.When the concentration of GA3 was 0.5 mg/L,seed germination rate reached 55.56% and germination energy 34.44%.[Conclusion]Cold stratification,100.0 mg/L GA3 and 100.0 mg/L NAA soaking treatments cau raise seed germination rate of R.chinensis.Appropriate seeding matrix and culture medium can promote seed germination of R.chinensis.
Rosa chinensisseed germinationgerm-free germinationgermination rate