8种海南热带水果的抗氧化活性比较分析
Antioxidant activity comparison of eight kinds of tropical fruits in Hainan
吕金慧 1郑中兵 2吴涛 3孙娅辉 1陈萍1
作者信息
- 1. 海南大学南繁学院(三亚南繁研究院),海南三亚 572025;海南大学热带农林学院,海南海口 570228
- 2. 海南大学南繁学院(三亚南繁研究院),海南三亚 572025
- 3. 海南大学热带农林学院,海南海口 570228
- 折叠
摘要
[目的]测定海南8种热带水果的抗氧化活性、总酚和总黄酮含量,比较8种水果抗氧化活性和抗氧化物质含量的差异,为人们食用热带水果提供一定的营养价值参考,也为抗氧化活性较强的热带水果相关产品的开发利用提供参考依据.[方法]以人心果、莲雾、山竹、榴莲、蛋黄果、西番莲、番荔枝和菠萝蜜8种热带水果为研究对象,利用1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除能力法、铁离子抗氧化能力(FRAP)法和2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)阳离子自由基清除能力法分别测定其抗氧化活性,采用福林酚(Folin-Ciocalteu)法测定总酚含量,AlCl3显色法测定总黄酮含量;并对3种抗氧化活性测定方法与2种抗氧化物质含量进行相关分析,对抗氧化活性测定结果进行优劣解距离(TOPSIS)法综合分析.[结果]利用DPPH法和FRAP法测得抗氧化活性最强的是蛋黄果(178.62和140.83 μmol Trolox/g),最弱的是人心果;ABTS法测得抗氧化活性最强的是山竹(157.06 μmol Trolox/g),最弱的是人心果;同时测得蛋黄果的总酚含量(3.80%)和总黄酮含量(7.60%)在8种水果中最高,而人心果的总酚含量(0.09%)最低,榴莲的总黄酮含量(1.07%)最低;3种测定方法与2种抗氧化物质的相关分析结果表明,DPPH法与总酚和总黄酮含量的相关系数达0.81以上,FRAP法与总酚和总黄酮含量的相关系数达0.90以上;利用TOPSIS法进行综合分析,抗氧化活性排序为蛋黄果>山竹>莲雾>西番莲>番荔枝>菠萝蜜>榴莲>人心果.[结论]8种热带水果均具有一定的抗氧化活性,以蛋黄果的抗氧化活性较强,人心果的抗氧化活性较弱.
Abstract
[Objective]The antioxidant activity and the contents of total phenolics and flavonoids of eight kinds of tropical fruits in Hainan were determined,and the differences of antioxidant activity and antioxidant substances in eight kinds of fruits were compared,so as to provide certain nutritional reference value for people to eat tropical fruits,it also provided reference for the development and utilization of tropical fruit related products with strong antioxidant activity.[Method]Eight tropical fruits including Manilkara zapota,Syzygium samarangense,Garcinia mangostana,Durio zibet-hinus,Lucuma nervosa,Passiflora edulis,Annona squamosa and Artocarpus heterophyllus were studied in this experi-ment.The antioxidant activity of the samples was determined by 1,1-dipheny1-2-picrylhydrazyl(DPPH)free radical method,ferric reducing antioxidant power(FRAP)and diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)cation free radical scavenging activity method.The total phenolics content was determined by the Folin-Ciocalteu method,and the total flavonoids content was determined by AlCl3 chromotest method.The correlation analysis of three different determination methods and two antioxidant substances were carried out,and the results of antioxidant activity were analyzed by technique for order preference by similarity to ideal solution(TOPSIS)method.[Result]The most effective antioxidant activity measured by DPPH method and FRAP method was L.nervosa(178.62 and 140.83 μmol Trolox/g),and that of M.zapota was the weakest.The highest antioxidant activity was found in G.mangostana(157.06 μmol Trolox/g)and that of M.zapota was the weakest by ABTS method.At the same time,the content of total phenolics(3.80%)and total flavonoids(7.60%)in L.nervosa was the highest among the eight kinds of fruits,the con-tent of total phenolics in M.zapota was the lowest(0.09%),and the content of total flavonoids in D.zibethinus was the lowest(1.07%).Correlation analysis of three different determination methods and two antioxidant substances was found,the correlation coefficient between DPPH method and total phenolics and total flavonoids contents reached more than 0.81,the correlation between FRAP method and total phenolics and total flavonoids contents was more than 0.90.The re-sults were analyzed by the TOPSIS method,and the antioxidant activity was ranked as:L.nervosa>G.mangostana>S.sa-marangense>P.edulis>A.squamosa>A.heterophyllus.>D.zibethinus>M.zapota.[Conclusion]All the eight kinds of tropical fruits have antioxidant activity,the antioxidant activity of L.nervosa is strong,and that of M.zapota is weak.
关键词
热带水果/抗氧化活性/总酚/总黄酮/TOPSIS法/海南Key words
tropical fruit/antioxidant activity/total phenolics/total flavonoids/TOPSIS method/Hainan引用本文复制引用
出版年
2023
国家科技期刊平台
NSTL
维普
万方数据