[Objective]The distribution and characteristics of microsatellite sequences were searched and analyzed in the genome of Mastacembelus armatus,which could provide a theoretical basis for the evaluation of M.armatus germ-plasm resources and marker-assisted breeding.[Method]Based on genomic sequence data of M.armatus,microsatellite loci were searched by MISA software and their distribution and composition characteristics in the genome of M.armatus were evaluated by bioinformatics analysis.[Result]19020 microsatellites were obtained from genome of M.armatus in-cluding 16025 perfect microsatellites.The number of microsatellites distributed on chromosome 2 was the most,and the least was on chromosome 24,which accounting for 10.26%and 1.94%of the total number of microsatellites respectively.Mononucletide was the most abundant microsatellite while hexanucleotide was the lowest.The first four types of perfect microsatellites in the genome of M.armatus were A/T,AC/GT,G/C and AG/CT,which accounting for 37.82%,29.57%,6.88%and 6.77%of the total number respectively.The range of the repeat times of motifs was 5-104 with the main repeat times including 5-6 and 10-11.The length interval of microsatellites was 10-192 bp,and the main length in-terval was 10-36 bp,which taking up 92.46%of the total number.The maximum number of microsatellites was 12 bp in length,which accounting for 15.22%of the total number of microsatellites.Pearson correlation analysis showed that there was extremely significantly negative correlation between the length and number of microsatellites for M.armatus(P<0.01).Functional annotation and analysis of GO showed that microsatellites genes were mainly enriched in three groups including biological process,molecular function and cellular component,and nucleus was the most significantly enriched term.[Conclusion]The microsatellites obtained based on the genome of M.armatus have high availability and polymor-phism potential,which can provide genetic data for the efficient development of microsatellite molecular markers.
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