摘要
[目的]探明玻璃轻石构建序批式生物膜反应器(SBBR)在水产养殖污水中的脱氮性能,为生物膜法处理水产养殖污水工艺中高效、低廉的载体材料选择提供模型及数据参考.[方法]以玻璃轻石为载体构建SBBR模型,利用水产养殖池底泥进行挂膜,并进行玻璃轻石不同孔径(3～8、2～3、1～2和0.1～1.0 mm)、粒径(10～20、20～30和30～50 mm)和填充率(20%、30%和40%)脱氮性能优化试验,以获得最佳脱氮性能SBBR参数;以沸石、火山石、活性炭和SiO2颗粒为对照,对比各载体材料生物膜中的挥发性悬浮物(VSS)和细胞外聚合物(EPS)含量,比较不同载体材料对疏水性蛋白的吸附能力.[结果]玻璃轻石以孔径1～2 mm、粒径10～20 mm、填充率20%构建SBBR的除氮性能最优,第30 d时氨氮、亚硝氮和总氮的去除率分别可达(90.61±0.30)%、(99.45±0.08)%和(94.04±0.14)%,玻璃轻石上的VSS含量为3.87±0.11 g/L,EPS含量为80.99±0.05 mg/g-VSS,显著高于其他载体材料处理(P<0.05,下同),同时EPS中具有更高的蛋白/多糖(PN/PS)比值,有利于生物膜的形成;除氮细菌吸附试验结果,玻璃轻石可吸附(58.08±0.15)%的牛血清白蛋白(BSA),显著高于沸石(41.72±0.40)%、火山石(25.34±0.53)%、活性炭(20.68±0.13)%和SiO2颗粒(15.56±0.38)%.[结论]以玻璃轻石构建的SBBR应用于水产养殖污水处理具有良好的脱氮性能,且具有较好的除氮细菌吸附能力,是良好的生物膜技术修复水产养殖污水的载体材料.
Abstract
[Objective]The denitrification performance of glass pumice constructed sequencing batch biofilm reactor(SBBR)in aquaculture sewage was studied,which provided model and data reference of an efficient and low-cost carrier choice for biofilm treatment of aquaculture sewage.[Method]The SBBR was constructed with glass pumice as the carrier,using aquaculture pond sediment to hang film,and the pore size(3-8,2-3,1-2 and 0.1-1.0 mm),particle size(10-20,20-30 and 30-50 mm)and filling rate(20%,30%and 40%)of the glass pumice were optimized for the optimal construction pa-rameters of the SBBR.Taking zeolite,volcanic stone,activated carbon,and SiO2 particles as controls,the volatile sus-pended solid(VSS)and extracellular polymeric substances(EPS)of each carrier material were compared,the adsorption capacity of different materials for more hydrophobic proteins was compared.[Result]The SBBR constructed with 1-2 mm pore size,10-20 mm particle size,and 20%filling rate had the best nitrogen removal performance.On day 30,the removal rates of ammonia nitrogen,nitrous nitrogen and total nitrogen concentration could reach(90.61±0.30)%and(99.45±0.08)%and(94.04±0.14)%,the content of VSS on glass pumice was 3.87±0.11 g/L,and the content of EPS was 80.99±0.05 mg/g-VSS,which were significantly higher than other groups(P<0.05,the same below),and EPS had a higher pro-tein/polysaccharide(PN/PS)ratio,which was conducive to the formation of biofilms.In the adsorption experiment of nitrogen-removing bacteria,glass pumice adsorbed(58.08±0.15)%of bovine serum albumin(BSA),which was signifi-cantly higher than that of zeolite(41.72±0.40)%,volcanic rock(25.34±0.53)%,activated carbon(20.68±0.13)%and SiO2 particles(15.56±0.38)%.[Conclusion]The application of SBBR constructed with glass pumice in aquaculture sewa-ge treatment has good denitrification ability,and excellent adsorption capacity of nitrogen removal bacteria,which is a good carrier material for biofilm technology to repair aquaculture sewage.
基金项目
国家重点研发计划项目(2022YFE0203900)
上海市协同创新中心基金(ZF1206)
上海海洋大学—江苏晶瑞特环保新材料有限公司联合研发计划项目(D-8006-20-0106)
维普
万方数据