首页|不同年限三七根系丛枝菌根真菌多样性及其与土壤理化性质的相关分析

不同年限三七根系丛枝菌根真菌多样性及其与土壤理化性质的相关分析

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
[目的]明确不同年限三七根系丛枝菌根真菌(AMF)多样性、群落结构差异及其与根际土壤理化性质的关系，为AMF在缓解三七连作障碍中的应用提供参考依据。[方法]以1年生、2年生和3年生三七根系及根际土壤为试验材料，采用显微形态法观察并测定根系AMF侵染率，通过Illumina高通量测序技术分析不同年限三七根系AMF多样性和群落结构差异，探讨三七根系AMF多样性和相对丰度与根际土壤理化性质的相关性。[结果]三七根系AMF侵染率在83%～95%，且随着种植年限延长，根际土壤的碱解氮、全氮和全磷含量逐渐减少，有效磷和速效钾含量先降低后升高，土壤酸化加强。通过高通量测序技术在三七根系中共检测到55个OTUs，排除不可归类，分属于球囊霉属(Glomeromycetes)的4目6科7属19种，其中球囊霉属(Glomus)占比最高(达43。99%)。多样性分析结果发现，对比其他2个年限，3年生三七根系OTUs最少，且多样性和丰富度最低。偏最小二乘法判别分析(PLS-DA)结果表明，不同年限三七根系AMF群落结构差异明显，其中球囊霉属是1年生和3年生三七根系的优势属(占比分别为32。31%和53。68%)，类球囊霉属(Paraglomus)为2年生三七的优势属(占比50。39%)。相关分析结果发现，三七根系AMF的ACE和Chao指数与根际土壤全磷含量呈显著正相关(P<0。05，下同)，与有效磷和速效钾含量呈显著或极显著(P<0。01)负相关。[结论]不同年限三七根系AMF的丰富度和群落结构存在明显差异，随种植年限增加，三七根系AMF丰富度和OTUs逐渐降低，但球囊霉属相对丰富度逐渐上升。在三七根际土壤中，有效磷和速效钾含量是影响其根系AMF群落结构的主要因素。
Correlation analysis between diversity of arbuscular mycorrhi-zal fungi in roots and soil physicochemical properties of Panax notoginseng at different ages
[Objective]To clarify the relationship between arbuscular mycorrhizal fungi(AMF)diversity,community structure and physicochemical properties of rhizosphere soil of Panax Notoginseng(Burk.)F.H.Chen in different years,and provide reference for the application of AMF in alleviating the obstacles of continuous cropping of P.Notoginseng.[Method]In this study,the root system and rhizosphere soil of P.Notoginseng aged one,two and three year-old were used as experimental materials.The AMF infection rate of root system was observed by using the microscopic morphology ob-servation method.The differences of AMF diversity and community structure of the P.Notoginseng root system were ana-lyzed using Illumina high-throughput sequencing.Pearson correlation coefficient method was used to reveal the correla-tion between relative abundance of P.Notoginseng roots AMF diversity and physicochemical properties of rhizosphere soil.[Result]The results showed that the AMF infection rates of P.notoginseng root ranged from 83%to 95%.With the extension of planting years,the contents of alkali-hydrolyzed nitrogen,total nitrogen and total phosphorus in rhizosphere soil gradually decreased,the contents of available phosphorus and available potassium first decreased and then increased,and the soil acidification was strengthened.A total of 55 OTUs were detected in the root system of P.notoginseng by high-throughput sequencing.Excluding the unclassified groups,these OTUs belonged to 19 species,4 orders,6 families,7 gen-era of Glomeromycetes,and Glomus accounted for the highest proportion(43.99%).The results of the diversity analysis showed that the three-year-old P.notoginseng had the least number of root OTUs and the lowest diversity and richness compared to the one-year-old and two-year-old P.notoginseng.Partial least squares discriminant analysis(PLS-DA)showed that the AMF community structure of P.notoginseng root system was different in different years samples,and Glo-mus was the dominant genus of oneyear-old and there-year-old root system(proportions were 32.31%and 53.68%),while Paraglomus was the dominant genus of two-year-old root system(proportion 50.39%).The results of correlation analysis showed that the ACE and Chao indexes of AMF of P.notoginseng root was significantly positively correlated with total phosphorus in rhizosphere soil(P<0.05),and significantly or extremely significantly(P<0.01)negatively correlated with available phosphorus and available potassium.[Conclusion]There are obvious differences in the abundance and commu-nity structure of AMF in the root system of P.notoginseng in different years.With the increase of planting years,the abun-dance and OTUs of AMF in the root system of P.notoginseng gradually decrease,but the relative abundance of Myco-sphaerella galloprovincialis gradually increases.In the inter-root soil of P.notoginseng,the contents of effective phospho-rus and quick-acting potassium were the main factors affecting the community structure of AMF in its root system.

Panax notoginseng(Burk.)F.H.Chenroot systemarbuscular mycorrhizal fungicommunity struc-turesoil physicochemical propertiescorrelation

杨双琳、邓利娟、黄博、王益锋、黄勇然、肖文度、郑洵、苏源、任禛、尹敏

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昆明学院农学与生命科学学院/云南省高校生物炭工程研究中心,云南昆明 650214

云南大学医学院,云南昆明 650091

三七 根系 丛枝菌根真菌 群落结构 土壤理化性质 相关性

云南省万人计划青年拔尖人才专项云南省地方高校联合专项重点项目云南省教育厅科学研究基金项目云南云天化股份有限公司研发中心项目

YNWR-QNBJ-2020-096202101BA 070001-0352023Y0859HX2023011

2023

10.3969/j.issn.2095-1191.2023.11.009

南方农业学报
广西壮族自治区农业科学院

南方农业学报

CSTPCDCSCD北大核心
影响因子：0.83
ISSN：2095-1191
年,卷(期)：2023.54(11)
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