超声波辅助提取地涌金莲多酚及其抗氧化活性分析
Ultrasonic-assisted extraction of polyphenols from Musella lasiocarpa(Franch.)and antioxidant activity of the extracted polyphenols
韩明冲 1周旭红 2李南 3茹月蓉 1镡思怡 1王振兴1
作者信息
- 1. 西南林业大学生命科学学院,云南昆明 650224
- 2. 云南中医药大学科技处,云南昆明 650500
- 3. 昆明市粮油饲料产品质量检验中心,云南昆明 650041
- 折叠
摘要
[目的]采用响应面法优化超声波辅助提取地涌金莲多酚的工艺条件,评价其抗氧化活性,以期为地涌金莲的开发利用提供参考依据.[方法]以地涌金莲花瓣为原料,在单因素试验的基础上,依据Box-Behnken中心组合原理选择超声波功率(X1)、提取温度(X2)、提取时间(X3)和料液比(X4)为试验因素,多酚提取量(Y)为响应值进行响应面试验,优化得到超声波辅助提取的最佳工艺条件;并以清除1,1-二苯基-2-三硝基苯肼(DPPH)自由基和2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)阳离子自由基的能力及总抗氧化能力(FRAP)为指标评价地涌金莲多酚提取物的抗氧化活性.[结果]各因素对地涌金莲多酚提取量的影响排序:X2>X3>X1>X4;X1X2、X1X3的交互作用对多酚提取量的影响极显著(P<0.01),X2X3的交互作用对多酚提取量的影响显著(P<0.05).模型方程优化为Y=100.73-4.87X1X2-5.03X1X3-4.09X2X3-11.13X12-9.06X22-12.05X32-10.46X42,此时的地涌金莲多酚提取最佳工艺参数为超声波功率360 W、提取温度55 ℃、提取时间30 min、料液比1∶20(g/mL),在此条件下的地涌金莲多酚提取量为10.51 mg/g,与理论值(10.08 mg/g)接近.抗氧化试验结果显示,地涌金莲多酚提取物清除DPPH自由基和ABTS阳离子自由基的半抑制浓度(IC50)分别为20.03和39.68 μg/mL,FRAP值为47.540 μg/mL,且抗氧化能力高于槲皮素.[结论]超声波辅助提取的地涌金莲多酚具有良好的抗氧化活性,可开发为一种安全、天然、可食用的抗氧化剂.
Abstract
[Objective]The response surface method was used to optimize the process conditions of ultrasound-assisted extraction of polyphenols from Musella lasiocarpa(Franch.),and the antioxidant activities of M.lasiocarpa poly-phenol extract(MLPE)were further evaluated,to provide theoretical support for further development and utilization of M.lasiocarpa.[Method]Ultrasonic power(X1),extraction temperature(X2),extraction time(X3)and solid-liquid ratio(X4)were selected as experimental factors,and polyphenol yield(Y)was the response value,response surface methodol-ogy was conducted to optimize conditions of ultrasound-assisted extraction from M.lasiocarpa petal on the basis of single factor experiments.The antioxidant activity of the polyphenol extract was evaluated by the scavenging ability of 1,1-diphe-nyl-2-trinitrophenylhydrazine(DPPH)free radicals and 2,2-diazo-bis(3-ethyl-benzothiazole-6-sulfonic acid)diamiam-monium salt(ABTS)and total antioxidant capacity(FRAP).[Result]The effect of each factor on the extraction rate of polyphenol from the M.lasiocarpa was X2>X3>X1>X4.The effect of interaction between X1X2 and X1X3 on the polyphenol extraction rate were extremely significant(P<0.01),while the effect of interaction effect of X2X3 was signifi-cant(P<0.05).The optimized model equation was Y=100.73-4.87X1X2-5.03X1X3-4.09X2X3-11.13X12-9.06X22-12.05X32-10.46X42,and the optimal extraction process parameter for polyphenols from the M.lasiocarpa were ultrasonic power of 360 W,extraction temperature of 55℃,extraction time of 30 min,and liquid-solid ratio of 1∶20(g/mL).Under these op-timal conditions,the extraction amount of polyphenols from M.lasiocarpa was 10.31 mg/g,which was consistent with the theoretical value of 10.08 mg/g.Results from antioxidant assays showed that the half maximal inhibitory concentration(IC50)values of DPPH and ABTS radical scavenging activity were 20.03 and 39.68 μg/mL,respectively,with a FRAP values of 47.54 μg/mL,antioxidant ability were higher compared to quercetin.[Conclusion]This study indicated that MLPE extracted by ultrasonic assisted extraction has good antioxidant activity,which can be developed into a edible,safe and natural antioxygen.
关键词
地涌金莲/多酚/超声波辅助提取/抗氧化活性Key words
Musella lasiocarpa(Franch.)/polyphenols/ultrasonic assisted extraction/antioxidant activities引用本文复制引用
基金项目
中央引导地方科技发展资金科技创新基地建设项目(202207AB110015)
云南省教育厅科学研究基金项目(2021Y271)
云南省大学生创新创业训练计划项目(2020)()
西南林业大学生物学质量工程项目(503190106)
Training Project of College Students'Innovation and Entrepreneurship in Yunnan(2020)()
出版年
2023
国家科技期刊平台
NSTL
万方数据