gga-miR-30d-5p表达分析及其对鸡脂肪沉积的调控作用
Expression analysis of gga-miR-30d-5p and its regulation of fat deposition in chickens
黄华云 1李瑞瑞 2张子晓 3王钱保 1黄正洋 1李春苗 1赵振华1
作者信息
- 1. 江苏省家禽科学研究所,江苏扬州 225125
- 2. 江苏省家禽科学研究所,江苏扬州 225125;佛山科技大学,广东佛山 528011
- 3. 江苏省家禽科学研究所,江苏扬州 225125;河北科技师范大学,河北秦皇岛 066600
- 折叠
摘要
[目的]分析gga-miR-30d-5p在鸡腹脂和肌内脂肪沉积中的作用,为进一步阐明鸡腹脂和肌内脂肪沉积的分子机制提供理论依据.[方法]实时荧光定量PCR检测gga-miR-30d-5p在S3系鸡和隐性白羽鸡不同发育时期不同组织中的表达,转染gga-miR-30d-5p mimics和inhibitor,观察脂肪细胞脂肪沉积的变化.[结果]gga-miR-30d-5p在心脏、肝脏、脾脏、皮脂、腹脂、胸肌、肾脏、卵巢和腿肌组织中均有表达.在腹脂组织中,gga-miR-30d-5p在0周龄时的表达存在显著的品种差异(P<0.05,下同),且在0周龄时的表达均显著高于其他周龄;肝脏组织中,gga-miR-30d-5p在0、14和16周龄的表达存在显著的品种差异,gga-miR-30d-5p在S3系鸡中的表达随着发育时期的增长逐渐升高,16周龄的表达显著高于其他周龄;在隐性白羽鸡,16周龄的表达最高且显著高于8周龄;腿肌组织中,gga-miR-30d-5p在2周龄时的表达存在显著的品种差异性.在脂肪细胞中,gga-miR-30d-5p在诱导分化4 d后的表达均显著高于增殖期;转染gga-miR-30d-5p mimics,脂肪细胞脂滴沉积显著升高;转染gga-miR-30d-5p inhibitor脂肪细胞脂滴沉积能力显著下降.靶基因GO和KEGG分析结果提示,SOCS3和PGP可能是gga-miR-30d-5p调控脂肪沉积的预测靶基因.[结论]gga-miR-30d-5p在鸡的肝脏、腹脂和腿肌组织中表达存在显著的品种差异,转染gga-miR-30d-5p mimics/inhibitor脂肪细胞脂滴沉积显著升高/降低.gga-miR-30d-5p具有促进鸡腹脂和肌内脂肪沉积的作用.
Abstract
[Objective]The purpose of the study was to analyze the effects of gga-miR-30d-5p on abdominal fat and in-tramuscular fat deposition of chicken,and to provide a theoretical basis for further elucidating the molecular mechanism of abdominal fat and intramuscular fat deposition in chickens.[Method]Real-time fluorescence quantitative PCR was used to detect the expression of gga-miR-30d-5p in different tissues of S3 line chickens and recessive white feather chick-ens at different developmental periods.The gga-miR-30d-5p mimics and inhibitor were transfected to observe the changes of fat deposition in adipocytes.[Result]The gga-miR-30d-5p was expressed in heart,liver,spleen,sebum,abdominal fat,chest muscle,kidney,ovary and leg tissues.In abdominal fat tissue,gga-miR-30d-5p expression at 0 week of age showed significant difference between breeds(P<0.05,the same below),and the expression at 0 week of age was signifi-cantly higher than that of other weeks of age.In liver tissues,there were significant breed differences in the expression of gga-miR-30d-5p at 0,14 and 16 weeks of age.In S3 line chickens,the expression of gga-miR-30d-5p increased gradually with the growth of developmental period,and the expression at 16 weeks of age was significantly higher than that of other weeks of age.In recessive white chickens,the expression was the highest at16 weeks of age significantly higher than that of 8 weeks.In leg muscle tissues,gga-miR-30d-5p expression at 2 weeks of age showed significant difference between breeds.In adipocyts,the expression of gga-miR-30d-5p was significantly higher than that in the proliferation period after 4 d after inducing differentiation.Lipid drop deposition was significantly increased in adipocytes after transfection with gga-miR-30d-5p mimics.Lipid drop deposition was significantly decreased in adipocytes after transfection with gga-miR-30d-5p inhibitor.The enrichment analysis results of GO and KEGG of target genes showed that SOCS3 and PGP may be the predicted target genes of gga-miR-30d-5p to regulate fat deposition.[Conclusion]The expression of gga-miR-30d-5p in chicken liver,abdominal fat and leg muscle tissues shows significant difference between breeds.Lipid drop deposition in adipocytes is significantly increased/decreased after transfection with gga-miR-30d-5p mimics/inhibitor.The gga-miR-30d-5p has the effects of promoting abdominal fat and intramuscular fat deposition in chickens.
关键词
gga-miR-30d-5p/鸡/腹脂脂肪/肌内脂肪/脂肪沉积Key words
gga-miR-30d-5p/chicken/abdominal fat/intramuscular fat/fat deposition引用本文复制引用
基金项目
现代农业产业技术体系建设专项(CARS-41-Z05)
江苏省种业振兴揭榜挂帅项目(JBGS2021109)
江苏省种业振兴揭榜挂帅项目(JBGS2021029)
国家家养动物种质库建设项目(资源库2023)
Jiangsu Seeds Industry Revitalization Project(JBGS2021109)
Jiangsu Seeds Industry Revitalization Project(JBGS2021029)
Construction Project of National Domestic Animal Germplasm Bank(Ziyuanku 2023)
出版年
2023
国家科技期刊平台
NSTL
万方数据