茄子青枯病抗性遗传效应分析
Genetic effect of eggplant resistance to bacterial wilt
郑洁明 1李永强 1吴元彩 1王鹏 1阳燕娟 1于文进1
作者信息
- 1. 广西大学农学院,广西南宁 530004
- 折叠
摘要
[目的]解析茄子青枯病抗性遗传规律,为选育抗病杂交组合、优势育种以及挖掘相关抗病基因提供理论支持与技术指导.[方法]以4份茄子种质(Y23、NO21、JA02和SG19)为亲本,通过Griffing完全双列杂交方法对各组合青枯病抗性进行配合力分析;分别构建2个杂交组合SG19×Y23和B1×BC03的6世代遗传群体(P1,P2,F1,F2,BC1P1和BC1P2),采用数量性状主基因+多基因世代联合分析法,对各世代青枯病抗性进行遗传模型分析.[结果]茄子青枯病抗性一般配合力远高于特殊配合力,说明抗性以加性效应为主,其次是非加性效应,受到细胞质基因遗传的影响较小;广义遗传力为69.8%,狭义遗传力为63.3%,说明茄子青枯病抗性的遗传不仅受遗传效应的影响,同时也受到环境效应的影响;抗青枯病的优良杂交组合为Y23×NO21、NO21×Y23;杂交组合SG19×Y23和B1×BC03的抗性均符合MX2-ADI-ADI遗传模型,即由2对加性—显性—上位性效应主基因+加性—显性—上位性效应多基因控制,以加性效应为主,感病表现为不完全显性,BC1P2群体和F2群体以主基因遗传为主,主基因遗传率为77.1%～88.4%,BC1P1群体以多基因遗传为主,多基因遗传率在35.3%～75.1%.通过发病时期作为病情评价标准估算出的最优遗传模型与通过病级分布估算出的最优模型一致.[结论]在青枯病抗病育种过程中,应注意加性效应的利用,尽可能选用青枯病抗性强的亲本进行杂交育种;在后续开展基因定位过程中,可利用2对主基因+多基因控制青枯病抗性的理论作为抗性基因定位的依据,避免抗性基因挖掘的盲目性.
Abstract
[Objective]The genetic law of bacterial wilt resistance in eggplant was analyzed to provide theoretical ba-sis for cross breeding and disease resistance breeding.[Method]Using 4 eggplant germplasm as parents,the combining ability analysis of bacterial wilt resistance in each combination was conducted by Griffing complete diallel crossing method;At the same time,the six generation genetic populations(P1,P2,F1,F2,BC1P1,BC1P2)of two cross combinations"SG19×Y23"and"B1×BC03"were constructed respectively.The combined generation analysis method of major gene+ multiple gene for quantitative traits was used to analyze the genetic model of bacterial wilt resistance in each generation.[Result]The general combining ability of resistance to eggplant bacterial wilt was much higher than that of special com-bining ability,indicating that the resistance was mainly additive effect,followed by non-additive effect,and was less in-fluenced by cytoplasmic genetic inheritance.The generalized heritability was 69.8%,and the narrow heritability was 63.3%,and the resistance was partly influenced by environment.The best cross combinations of resistance to bacterial wilt were"Y23×NO21"and"NO21×Y23".The resistance of the hybrid combination"SG19×Y23"and"B1×BC03"was in line with the"MX2-ADI-ADI"genetic model,that is,it was controlled by two pairs of additive-dominance-epistatic effect major genes + additive-dominance-epistatic effect multiple genes.The additive effect was dominant,and the dis-ease susceptibility showed incomplete dominance.The BC1P2 and F2 populations were mainly inherited by major genes,with a heritability rate of 77.1%to 88.4%.The BC1P1 population was mainly inherited by multiple genes,with a heritabil-ity rate of 35.3%to 75.1%.The optimal genetic model estimated by the onset period was consistent with the optimal model estimated by the disease grade distribution.[Conclusion]In the breeding process of bacterial wilt resistance,attention should be paid to the use of additive effect,as far as possible to select strong bacterial wilt resistance parents for cross breeding;The theory of controlling bacterial wilt resistance with two pairs of major genes and multiple genes can be used as the basis for resistance gene mapping to carry out subsequent gene mapping related work to avoid blindness of resis-tance gene mining.
关键词
茄子/青枯病/遗传分析/双列杂交/主基因+多基因Key words
Eggplant/Bacterial wilt/Genetic analysis/Diallel crossing/Major gene+polygene引用本文复制引用
基金项目
国家自然科学基金项目(31660568)
广西科技重大专项(桂科AA22068088-2)
出版年
2023
国家科技期刊平台
NSTL
万方数据