Composition,diversity and function prediction of the gut bacterial community in different developmental stages of Eocanthecona furcellata(Wolff)
[Objective]The purpose of the study was to analyze the composition and diversity of gut bacterial commu-nities in different developmental stages of the natural enemy insect Eocanthecona furcellata(Wolff),and to predict their functions,in order to provide a theoretical basis for a deeper understanding of the influence of gut bacteria on the growth,development and nutrient acquisition patterns of E.furcellata,as well as for the development of a novel biocontrol strate-gies for the pests.[Method]The gut bacterial genomic DNA was extracted from the first instar nymphs,second instar nymphs,fifth instar nymphs,7-day-old female and male adults of E.furcellata,respectively.The full-length of 16S rRNA sequence of E.furcellata gut bacteria was sequenced using the single-molecule real-time sequencing technique.The number of operational taxonomic unit(OTU)was counted,the composition and diversity of gut bacterial communities were analyzed,and the functions of gut bacteria were predicted by PICRUSt.[Result]A total of 202401 effective se-quences were obtained from 15 gut samples of E.furcellata,and clustered into 222 OTUs,of which 5 OTUs were shared by them.A total of 19 phyla,32 classes,62 orders,103 families,154 genera and 188 species bacteria were annotated.The dominant phylum,class,order,family,genus and species of the bacteria were Proteobacteria(72.22％),Gammaproteo-bacteria(72.21％),Enterobacterales(72.11％),Enterobacteriaceae(49.39％),Cedecea(49.24％),and C.neteri(49.24％),respectively.The results of the diversity and variance analysis revealed significant differences in the composition of the gut bacterial flora in different developmental stages of E.furcellata(P＜0.05,the same below).The linear discriminant analysis results showed that significantly dominant flora existed in the guts of first instar nymphs,second instar nymphs and 7-day-old female and male adults,while no significantly dominant flora existed in the gut of fifth instar nymphs(P＞0.05),and the dominant flora in the gut of different developmental stages varied at the level of 3 taxonomic catego-ries,namely,family,genus and species.KEGG metabolic pathway analysis revealed that the functions of the gut flora of E.furcellata were similar in different developmental stages,mainly involving metabolism(74.17％-75.59％)and environ-mental information processing(10.53％-11.03％).The secondary metabolic pathways were mainly global and overview mapping,sugar metabolism,and amino acid metabolism,etc.[Conclusion]Significant differences in the composition and diversity of gut bacterial community are observed in different developmental stages of E.furcellata,and the distribu-tion of dominant bacteria shows dynamic changes.
万方数据
维普
