Effects of hormones on seed germination of Euscaphis konishii
[Objective]The role of hormones in seed germination of Euscaphis konishii and its relationship with reac-tive oxygen species were explored to provide an important theoretical basis for its seed dormancy breaking technology and efficient seedling breeding technology.[Method]E.konishii seeds with 210 d natural stratified were used as materials,outer hard seed coat was removed,a 12 d germination experiment was conducted.The changes of germination rate,active oxygen,antioxidant substances,plant hormones,soluble sugar and soluble protein contens under pure water,different con-centrations of gibberellin(GA3)(20,40 and 60 μmol/L)and abscisic acid(ABA)(10,20 and 30 μmol/L)were measured,and germination morphology of seeds was observed.[Result]The germination rate of pure water treatment was 77.8％,the germination rate of GA,treatment was up to 82.2％,the germination rate of ABA treatment did not reach 50.0％,and the germination rate of pure water treatment with hard seed coat was only 17.8％.The three concentrations of GA3 treat-ments could effectively shorten the time when germination rate reached 50.0％(T50)and promote root and stem growth,but the final germination rate was not significantly different from that of pure water treatment(P＞0.05).ABA treatment could effectively inhibit the germination of E.konishii seeds.The higher the concentration of ABA,the stronger the inhibi-tion.Correlation analysis showed that the germination rate was significantly positively correlated with GA3,brassinolide(BR)and jasmonic(JA)at 8 and 12 d(P＜0.05,the same below),superoxide anion(O2·)was negatively correlated with BR and JA contents,and significantly positively correlated with catalase(CAT),ABA,ABA/GA3.[Conclusion]Adding GA3 can effectively shorten the time of the seed germination rate of E.konishii to reach 50％and promote the growth of root and stem.The accumulation of O2-·and ABA inhibit seed germination and the promotion of BR and JA on seed germi-nation in E.konishii may be related to the removal of O2-·.
万方数据
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