广西小花老鼠簕可培养细菌多样性及促生、防病、产酶活性分析
Diversity of culturable endophytic bacteria in Acanthus ebractea-tus of Guangxi and their growth-promoting,biocontrol effect and enzyme production activity
殷豆豆 1黄宏智 1宋超东 1刘睿 1梁颖 1陆君铭 1谢俊杰 1阎冰 2申乃坤 1张红岩1
作者信息
- 1. 广西民族大学海洋与生物技术学院/广西多糖材料与改性重点实验室,广西南宁 530006
- 2. 广西科学院广西红树林研究中心/广西红树林保护与利用重点实验室,广西北海 536007
- 折叠
摘要
[目的]分析广西小花老鼠簕可培养细菌多样性及促生、防病、产酶活性,为小花老鼠簕种群恢复及筛选可利用菌种资源提供参考依据.[方法]采用稀释涂布法对小花老鼠簕根、茎、叶及根际土壤可培养细菌进行分离、纯化;对菌株的16S rRNA序列进行PCR扩增,测序结果经EzBioCloud比对确定菌株的分类地位;通过功能培养基对菌株的促生[溶磷、固氮、产吲哚乙酸(IAA)和分泌铁载体]、防病(香蕉枯萎病)及产酶(蛋白酶、淀粉酶和纤维素酶)活性进行分析.[结果]从小花老鼠簕根、茎、叶及根际土壤共筛选获得细菌159株,根据菌落形态及16S rRNA测序结果排除重复后为65株,隶属于8目7科14属,其中芽孢杆菌属(Bacillus)为优势菌属,占分离菌株的64.62%,同时发现1株潜在新种(Gxun-T66),与已发现菌株相似性仅为98.49%;植物促生防病活性结果显示,溶磷菌株34株、固氮菌株31株、产IAA菌株20株、分泌铁载体菌株17株,其中北澳伯克霍尔德氏菌(Burkholderia territorii)Gxun-T33的溶磷和分泌铁载体能力最强;29株对香蕉枯萎病尖孢镰刀菌(Fusarium oxysporum f.sp.Cubense)有明显抑制作用,其中暹罗芽孢杆菌(Bacillus siamensis)Gxun-T25的抑菌效果最好,抑菌率达69.67%;产酶活性结果显示,产蛋白酶菌株56株,产淀粉酶49株,产纤维素酶48株,同时产3种酶活性的菌株有34株,占分离菌株的52.31%.[结论]红树植物小花老鼠簕富含微生物资源且具备促生、防病及产酶活性,同时发现1株潜在新种.
Abstract
[Objective]The aim was to analyze the diversity of culturable bacteria of Acanthus ebracteatus in Guangxi and their growth-promoting,biocontrol effect and enzyme production activity,so as to provide reference for the recovery of A.ebracteatus ilicifolius population and the screening of available strain resources.[Method]The culturable bacteria in the roots,stems,leaves and rhizosphere soil were isolated and purified by dilution coating method.The 16S rRNA se-quence of the strain was amplified by PCR,and the sequencing results were compared by EzBioCloud to determine the taxonomic status of the strain.The growth-promoting[phosphate solubilization,nitrogen fixation,indoleacetic acid(IAA)production and siderophore secretion],disease prevention(banana fusarium wilt)and enzyme production(protea-se,amylase and cellulase)activities of the strains were analyzed by functional medium.[Result]A total of 159 strains of bacteria were screened from the roots,stems,leaves and rhizosphere soil of A.ebracteatus.According to the colony mor-phology and the 16S rRNA sequencing results,65 strains were excluded after duplication,belonging to 8 orders,7 fami-lies and 14 genera.Among them,Bacillus was the dominant genus,accounting for 64.62% of the isolated strains.At the same time,a potential new species(Gxun-T66)was found,and the similarity with the discovered strains was only 98.49% .The results of plant growth-promoting and biocontrol effect showed that 34 strains were phosphorus-solubilizing strains,31 strains were nitrogen-fixing strains,20 strains were IAA-producing strains and 17 strains were siderophore-secreting strains.Among them,strain Burkholderia territorii Gxun-T33 had the strongest ability to dissolve phosphorus and to secrete siderophore;29 strains had obvious inhibition effect on Fusarium oxysporum f.sp.Cubense,among which strain Bacillus siamensis Gxun-T25 had the best inhibition effect,with an inhibition rate of 69.67% .The results of the en-zyme activity showed that there were 56 protease-producing strains,49 amylase-producing strains,48 cellulase-producing strains and 34 strains producing three kinds of enzyme activities at the same time,accounting for 52.31% of the isolated strains.[Conclusion]The mangrove plant A.ebracteatus is rich in microbial resources and has the activities of promoting growth,biocontrol effect and enzyme production.At the same time,a potential new species has been.
关键词
小花老鼠簕/可培养细菌/促生/拮抗菌/酶活性Key words
Acanthus ebracteatus/culturable bacteria/growth-promoting/antagonistic bacteria/enzyme activity引用本文复制引用
基金项目
国家自然科学基金项目(32060020)
广西重点研发计划项目(桂科AB21220020)
广西重点研发计划项目(桂科AB21196019)
广西大学生创新创业训练计划项目(S202210608156)
出版年
2024
国家科技期刊平台
NSTL
万方数据