Association between KAT2B gene polymorphisms and growth traits in Xiangsu hybrid pigs
[Objective]To understand the expression level of K(lysine)acetyltransferase 2B(KAT2B)gene in the tis-sues of Xiangsu hybrid pigs,to select the single nucleotide polymorphism(SNP)site of Xiangsu hybrid pigs,and to ana-lyze the association between gene polymorphism and growth traits,so as to provide reference for breeding new Xiangsu hybrid pigs.[Method]Xiangsu hybrid pigs were selected as the study object.Real-time fluorescence quantitative PCR was used to detect the relative expression of KAT2B gene in different tissues of 3 days and 6 months of age,and the SNP sites of KAT2B gene were detected by direct sequencing.Allele frequency,genotype frequency,heterozygosity(Ho),ex-pected heterozygosity(He),effective allele number(Ne)and average peptide information content(PIC)of SNP sites were measured.Chi-square test(χ2)was used to analyze whether the genotypes were in Hardy-Weinberg equilibrium state.Then analyzed the relationship between different genotypes and growth traits of Xiangsu hybrid pigs.[Result]Real-time fluorescence quantitative PCR results showed that KAT2B gene was expressed in 6 tissues of heart,liver,spleen,lung,kidney and longissimus dorsi muscle.Compared with 3 days of age,the relative expression level of liver and spleen was extremely significantly increased(P<0.01,the same below),while the relative expression level of lung was ex-tremely significantly decreased.Exon-2,Exon-14 and Exon-15 regions in KAT2B gene had one exon mutation,which were g.36682G>A,g.104540G>T and g.106651A>G,respectively.All the three SNPs sites were in moderate polymor-phism level and Hardy-Weinberg equilibrium.The dominant genotype of g.36682G>A site was GA type,and the allele frequency G>A;the dominant genotype of g.104540G>T site was GG genotype,and the allele frequency G>T;the domi-nant genotype of G.106651A>G site was AA,and the allele frequency was A>G.The results of association analysis showed that there was no significant difference between genotype and growth traits of g.36682G>A sites(P>0.05),and there was significant difference in chest depth between AA genotype and AG genotype at g.106651A>G(P<0.05).[Con-clusion]The KAT2B gene is expressed in all tissues of Xiangsu hybrid pigs and there are three SNPs sites(g.36682G>A,g.104540G>T and g.106651A>G),KAT2B gene can be used as a candidate gene related to growth traits of Xiangsu hy-brid pigs.
万方数据
维普
