Mechanism effects of mogroside Ⅲ on the alleviation of porcine oocyte aging
[Objective]The study aimed to verify whether mogroside Ⅲ had the effects to delay the aging process of porcine oocytes in vitro and provide reference for utilizing plant extracts to alleviate oocyte aging in vitro.[Method]Por-cine oocytes cultured in vitro for 44 h were used as research subjects.The effective concentration of mogroside Ⅲ for por-cine oocytes was screened by parthenogenetic activation(PA).Three treatment groups were set up:fresh oocytes(Fresh),aging oocytes(Aging),and aging oocytes supplemented with mogroside Ⅲ.Immunofluorescence staining and real-time fluorescence quantitative PCR were employed to detect mitochondrial content and distribution,adenosine tri-phosphate(ATP)content,mitochondrial membrane potential,reactive oxygen species(ROS)levels,DNA damage,antioxidant-related gene expression,and early apoptosis levels in porcine oocytes.[Result]Aging extremely significantly decreased the embryonic cleavage rate and blastocyst development rate of parthenogenetic activated embryos derived from porcine oocytes(P<0.01,the same below).The addition of mogroside Ⅲ effectively improved the embryonic cleavage rate and blastocyst development rate of aging oocytes,especially at 25.0 µmol/L.which was therefore determined as the effective concentration for mogroside Ⅲ.Compared with the Fresh group,the Aging group exhibited significant de-crease in mitochondrial fluorescence intensity(P<0.05,the same below),and extremely significant decrease in ATP con-tent,significant decrease in mitochondrial membrane potential,and extremely significant decrease in the relative expres-sion levels of antioxidant-related genes,while ROS levels,γ-H2AX fluorescence intensity,and Annexin-V positive rate were extremely significantly increased.However,the addition of 25.0 µmol/L mogroside Ⅲ effectively improved the above problems,indicating that mogroside Ⅲ could effectively alleviate the aging process of porcine oocytes.[Conclu-sion]Mogroside Ⅲ effectively delays the aging process of porcine oocytes by improving mitochondrial number and ATP content,restoring ROS balance,up-regulating the expression of antioxidant-related genes,and alleviating DNA damage and early apoptosis caused by the aging of porcine oocytes,thereby enhancing the in vitro developmental competence of porcine oocytes.
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