Effects of chlorantraniliprole seed dressing on bacterial commu-nity diversity and enzyme activity in maize rhizosphere soil
[Objective]The study aimed to clarify the effects of chlorantraniliprole suspension seed dressing on the structure and diversity of bacterial communities in maize rhizosphere soil,as well as the activity of major enzymes,pro-viding scientific basis for the use of chlorantraniliprole seed dressing in controlling maize pests.[Method]The field plot experiment was conducted using water seed dressing as the control(CK)and chlorantraniliprole suspension agent seed dressing as the treatment(T).High-throughput sequencing technology was used to determine the structure and diversity of the bacterial communities in maize rhizosphere soil at 15,35,and 60 d after sowing.The activities of catalase,su-crase,and urease in the maize rhizosphere soil were measured using enzyme assay kits.The responses of bacterial commu-nity structure,diversity,and enzyme activity in maize rhizosphere soil to chlorantraniliprole seed dressing were investi-gated.[Result]At 15 d after maize sowing,the number of bacterial classifications in the T treatment was lower than that in the CK,but at 35 and 60 d after sowing,it was higher than that in the CK.Chlorantraniliprole seed dressing increased the bacterial community abundance,but its impact gradually diminished over time.The dominant phyla in both CK and T treatments were Proteobacteria,Acidobacteriota,Bacteroidota,Chloroflexi and Verrucomicrobiota.At 15 and 35 d after sowing,the relative abundance of Proteobacteria and Bacteroidota in the maize rhizosphere soil in the T treatment in-creased,while the relative abundance of Acidobacteriota,Chloroflexi and Verrucomicrobiota decreased.The dominant ge-nus in both treatments was unclassified_Acidobacteriales.At 15 and 35 d after maize sowing,the relative abundance of unclassified_Acidobacteriales in the T treatment was lower than that in the CK,while at 60 d after sowing,it was higher than that in the CK.Using PICRUSt 2 to perform the functional prediction on the bacterial 16S rRNA in maize rhizosphere soil,the results showed that the functional genes of soil bacteria after chlorantraniliprole seed dressing treatment were mainly related to metabolism and energy production.Chlorantraniliprole seed dressing activated catalase and sucrase ac-tivities in maize rhizosphere soil,while it had no significant effect on soil urease activity(P＞0.05).Soil catalase activity significantly increased by 57.85％and 51.67％at 15 and 60 d after sowing respectively(P＜0.05,the same below).Soil sucrase activity significantly increased by 19.95％and 61.66％at 35 and 60 d after sowing respectively.At 15 and 35 d af-ter sowing,the relative abundance of Bacteroidota,Verrucomicrobiota,Planctomycetota and Patescibacteria showed a positive correlation with soil catalase activity.At 60 d after sowing,the relative abundance of Acidobacteriota,Gemmati-monadota,and Nitrospirota showed a positive correlation with both soil catalase and sucrase activities.[Conclusion]In the early stages following chlorantraniliprole seed dressing treatment,the diversity of the microbial community and soil enzyme activity in the maize rhizosphere soil can be affected.However,as time progresses and the maize grows,the im-pact on the bacterial community structure gradually decreases.Chlorantraniliprole seed dressing treatment can signifi-cantly increase catalase and sucrase activities in maize rhizosphere soil,and soil catalase and sucrase activities are closely correlated to the dominant bacterial phyla in the maize rhizosphere soil.
万方数据
维普
