[Objective]To study the composition of culturable bacteria in the gut of nymphs of the Eocanthecona fur-cellata(Wolff),and analyze the growth promoting characteristics of the strains,so as to provide scientific basis and re-search materials for the study of the relationship among the gut bacteria of E.furcellata,hosts and plants,as well as the development and utilization of probiotic.[Method]The traditional bacterial isolation and culture method was used to iso-late and purify the gut bacteria of nymphs of E.furcellata.The isolated gut bacteria were classified and identified by bacte-rial morphology,staining reaction,physiological and biochemical tests and 16S rDNA sequence analysis,and qualitative analysis of the growth promoting potential of the strains was conducted by determining their nitrogen fixation,phospho-rus solubilization,indoleacetic acid production and siderophore production ability.[Result]A total of 11 strains of bacte-ria were isolated from the gut of nymphs of E.furcellata,the 11 strains of gut bacteria included 8 Gram-positive bacteria and 3 Gram-negative bacteria strains.The cell morphology of 3 strains was short rod-shaped,1 stain was globular,and 7 strains were rod-shaped.and the 11 strains of gut bacteria bebonged to 2 phyla,2 classes,3 orders,4 families and 4 genera.The dominant phylum,class,order,family and genus were Firmicutes(85.67％),Bacilli(85.67％),Lactobacillales(75.80％),Enterococcaceae(75.80％),Enterococcus(75.80％)respectively.At the species level,E.faecalis(75.80％),Bacillus subtilis(9.17％),Morganella morganii(6.51％),Serratia nematodiphila(4.49％),S.marcescens(3.30％),B.thuringiensis(0.28％),B.velezensis(0.27％)and B.altitudinis(0.18％)were identified as abundant bacterial species.Plant promoting characterization of the 11 isolated strains showed that 2 strains of Serratia had the highest promotion potential with 4 plant growth promoting properties:nitrogen fixation,phosphorus solubilization,indoleacetic acid pro-duction and siderophore production,followed by 1 strain of Morganella with 3 plant growth promoting properties:phos-phorus solubilization,indoleacetic acid production and siderophore production.[Conclusion]The culturable bacterial species in the gut of nymphs of E.furcellata are relatively abundant,and 11 bacterial strains isolated from the gut have different degrees of growth promoting properties.
关键词
叉角厉蝽/可培养细菌/分离鉴定/细菌多样性/植物促生特性
Key words
Eocanthecona furcellata(Wolff)/culturable bacteria/isolation and identification/bacterial diversity/plant growth promoting characteristics
维普
万方数据