摘要
[目的]探究饥饿胁迫对花鲈幼鱼摄食行为的分子调控,为应对花鲈饥饿胁迫和解析饥饿胁迫对花鲈摄食行为的影响提供理论参考.[方法]以花鲈幼鱼为研究对象,饥饿处理后检测饥饿胁迫对花鲈幼鱼体质量和摄食率的影响及饥饿胁迫对摄食相关基因相对表达量的影响.饥饿再投喂处理后,实时荧光定量PCR检测饥饿再投喂对花鲈幼鱼摄食相关基因相对表达量的影响.[结果]短期饥饿和中长期饥饿处理后花鲈幼鱼体质量和增重率均显著降低(P<0.05,下同),食欲生成相关基因相对表达量均显著升高,厌食生成相关基因相对表达量均显著降低.短期饥饿再投喂花鲈幼鱼摄食率能恢复到正常水平,食欲生成相关基因npy、agrp、mch和galanin相对表达量逐渐降低,投喂3 d后恢复到对照组水平;厌食生成相关基因m-tor、pomc和α-msh相对表达量逐渐升高.长期饥饿再投喂花鲈幼鱼食摄食率不能恢复至正常水平,食欲生成相关基因npy、agrp、mch、galanin和厌食生成相关基因leptin、m-tor、pomc和α-msh相对表达量均逐渐升高.[结论]短期饥饿胁迫和中长期饥饿胁迫花鲈幼鱼体质量和增重率均显著下降,短期饥饿再投喂花鲈幼鱼摄食率能恢复至正常水平,食欲生成相关基因相对表达量逐渐降低,厌食生成相关基因(m-tor、pomc和α-msh)相对表达量逐渐升高;中长期饥饿再投喂花鲈幼鱼摄食率不能恢复至正常水平,食欲生成相关基因和厌食生成相关基因相对表达量均呈逐渐升高的变化趋势.
Abstract
[Objective]Investigating the molecular regulation of feeding behavior in juvenile Lateolabrax maculatus under starvation stress,to provide theoretical references for coping with starvation stress in L.maculatus and elucidating the impact of starvation stress on their feeding behavior.[Method]Using juvenile L.maculatus as subjects,investigated the effects of starvation stress on body mass and feeding rate,as well as the impact on the relative expression levels of feeding related genes.After the starvation followed by feeding treatment,real-time fluorescence quantitative PCR was employed to assess the influence of starvation and feeding on the relative expression levels of feeding-related genes in the juvenile L.maculatus.[Result]After both short-term and medium and long term starvation treatments,the body mass and body mass gain of juvenile L.maculatus was significantly reduced(P<0.05,the same below),with a significant increase in the relative expression levels of appetite generation genes and a significant decrease in the relative expression levels of anorexia generation genes.After short-term starvation followed by feeding,the feeding rate of juvenile L.maculatus could recover to normal level,with the relative expression levels of appetite generation genes npy,agrp,mch and galanin grodually decreased,returning to control group level after 3 d of feeding;the relative expression levels of an-orexia generation genes m-tor,pomc and α-msh gradually increased.In contrast,long-term starvation and followed by feeding did not allow the feeding rate of juvenile L.maculatus to return to normal levels,with the relative expression levels of both appetite-stimulating genes npy,agrp,mch and galanin and anorexia generation genes leptin,m-tor,pomc and α-msh gradually increased.[Conclusion]The body mass and body mass gain of juvenile L.maculatus is significantly decreased under short-term and long-term starvation stress,and the feeding rate of juvenile L.maculatus after short-term starvation followed by feeding returned to normal level.The expression levels of appetite generation genes gradually de-crease,and the expression levels of anorexia generation genes(leptin,m-tor,pomc and α-msh)first decrease and then in-crease.The feeding rate of juvenile L.maculatus under long-term starvation followed by feeding can not recover to the normal level,and the relative expression levels of appetite generation genes and anorexia generation genes grodually in-crease.
维普
万方数据