Medaka gsdf Regulates Differential Expression of Igf2bp2/Igf2bp3-m6A Reader in Male and Female Germ Cells
Knockout of gonadal soma-derived factor(gsdf),led to the abnormal proliferation of germ cells and sexual reversal of XY males to females.Differences in transcriptome data of wild-type XY testis,XX ovary,and gsdf-deficient XY giant testis or XY giant ovary showed that the expression level of igf2bp2(insulin-like growth factor 2 mRNA binding protein 2)was significantly higher in wild-type XY sperms than that in wild-type XX ovary and gsdf-deficient XY sperms and XY ovary,and confirmed by RT-qPCR,suggesting that igf2bp2 expression may be regulated by gsdf signal.The molecular structure of vertebrate igf2bp2 was highly homology evolutionary conserved.RT-qPCR showed that igf2bp2 mRNA was highly expressed in early embryogenesis,especially in the stage of gastrulation.The expression of igf2bp2 was in early embryogenesis and predominant testis in adulthood,suggesting that igf2bp2 may play an important role in early embryonic development,sex differentiation and spermatogenesis.Immunofluorescence assay revealed that the signal of positive reaction of anti-Igf2bp2 antibody in wild-type XY testis was stronger than that of XX ovary,gsdf-deficient XY testis or XY ovary.The presence or absence of gsdf signaling was positively correlated with igf2bp2 expression during gonadal developmental differentiation in medaka,thus may potentially promote igf2bp2 expression,which was opposite to the inhibition of igf2bp3 expression.gsdf-regulatory role of igf2bp2/igf2bp3 expression during medaka spermatogenesis may be a conserved molecular mechanism in vertebrate gonadal differentiation and spermatogenesis.
国家科技期刊平台
NSTL
万方数据
维普
