Shechuang-Kushen Decoction Inhibit Effect and Mechanism of Compound 48/80 on Itch in ICR Mice
Common Cnidium and Sophora flavescens are commonly used antipruritic herbs,but their combined effect on itch has not been fully elucidated.In this study,ICR mice were gavaged with Shechuang-Kushen Decoction,and the effect of the drug on the pruritus behavior of the mice was observed by a behavioral method.The changes in vascular permeability were observed by Evans Blue staining.The effect of the decoction on the release of cytokines from the mast cell line RBL-2H3 was evaluated by ELISA and Western Blotting,respectively.The results showed that the decoction inhibited pruritus behavior and increased vascular permeability,but did not affect the exploration ability or muscle tone of the mice.The decoction solution reduced the release of β-hexosaminidase,histamine,IL-31,IL-4,IL-13,and CXCL10,and inhibited the phosphorylation of the ERK protein in RBL-2H3 cells.This study demonstrated that Shechuang-Kushen Decoction inhibited compound 48/80-induced acute pruritus by inhibiting RBL-2H3 cell degranulation and the release of pruritus-related cytokines.
Common CnidiumSophora flavescensitchrelieve itching
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