Expression Profiling of Related Genes of Muscle Growth in Mice After Intramuscular Cardiotoxin Injection
In this study,male C57BL/6 mice were used as experimental animals to establish muscle injury model by cardiotoxin(CTX)injection.Structural alterations of skeletal muscle were observed by the HE staining,and the expressions profiling of related genes of skeletal muscle growth and differentiation were detected by real-time quantitative PCR(RT-qPCR),the untreated mice were used as the control.The results revealed that there was significant muscle histologic alteration and tibialis anterior muscle weight-to-length ratio was decreased by the single injection of 50 μL CTX(1 g/L)into the mouse tibialis anterior muscle after 4 days.The expression of related genes for muscle differentiation,MyOD1,MyOG,MYH1,MYH2,AMPK,and mTOR signaling pathway marker genes(PRKAA1,NOTCH1,AKT,PI3K,mTOR)in the tibialis anterior muscle decreased significantly compared with those of the untreated group.This study successfully established the mouse muscle injury model after intramuscular Cardiotoxin injection by morphological examination and detection of related genes expression.The research provided a theoretical basis for molecular regulatory mechanisms for muscle regeneration and treatment methods for muscle injury.
万方数据
维普
