Mechanism of Qingre Huatan Tongfu formula in treating ischemic stroke based on network pharmacology
Objective:To explore the potential mechanism of Qingre Huatan Tongfu formula(HTF)in the treat-ment of ischemic stroke(IS)based on network pharmacology and animal experiments.Methods:The active in-gredients of HTF were screened using TCMSP and BATMAN databases and the potential targets of these active ingredients were predicted.Disease-related targets were screened in GeneCards,OMIM,TTD,and Drugbank da-tabases.The protein interaction relationship was obtained using the String database.Key targets underwent GO and KEGG enrichment analysis using Metascape.The animal model was constructed for experimental verifica-tion.Neural function scores,2,3,5-triphenyltetrazolium chloride(TTC)staining,hematoxylin-eosin(HE)stain-ing,Nissl staining,terminal deoxynucleotidyl transferase dUTP nick end labeling(TUNEL)staining and immuno-histochemistry were used to verify the network pharmacological enrichment results.Results:By conducting net-work pharmacological analysis,the main active ingredients were predicted to include apigenin,quercetin,kaemp-ferol,and β-sitosterol.These ingredients acted on potential targets such as serine/threonine kinase 1(AKT1),in-terleukin-6(IL-6),tumor necrosis factor-α(TNF-α),transformation-associated protein 53(TP53)and other poten-tial targets.The key pathways involved included lipid and atherosclerosis,MAPK signaling pathway,PI3K-Akt signaling pathway,TNF signaling pathway,IL-17 signaling pathway,and hypoxia-inducible factor-1(HIF-1)sig-naling pathway,etc.The experimental results showed that HTF could significantly reduce the nerve function score and the volume of cerebral infarction in cerebral ischemia-reperfusion rats(P＜0.05),improve the survival of neurons(P＜0.05),and significantly reduce the cell apoptosis(P＜0.05).Conclusion:Based on network phar-macology and animal experiments,it is found that HTF can play a role in the treatment of IS through multiple components acting on multiple targets,involving various pathways such as anti-inflammation and anti-apoptosis.
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