Effect of short-term high alcohol intake on behavior and motor function of Tupaia belangeri chinensis
Objective:To investigate the effect of short-term high alcohol intake on the behavior and motor func-tion of Tupaia belangeri chinensis(T.b.chinensis),so as to lay the foundation for further neuroscience research.Methods:An alcohol binge-drinking model was established on T.b.chinensis by giving 50%ethanol solutions of various volumes.Twenty male T.b.chinensis were randomly divided into control,low-dose,medium-dose,and high-dose groups,with 5 individuals per group.Open field test and gait analysis were used to comprehensively evaluate the basic behavioral characteristics of T.b.chinensis before and after drinking.The total movement dis-tance,average speed,highest speed,number of crossings through the central area,gait linearity Δα,alternation co-efficient β,and step regularity coefficient σ were compared among the three dose groups.Results:The open field experiment showed that the total movement distance,the average speed and the highest speed of T.b.chinensis in the medium-and high-dose groups were significantly deceased in comparison of those in the control group(all P＜0.01),while the number of crossings through the central area was significantly decreased in all three dose groups(all P＜0.01).Among them,the changes were most obvious in the high-dose group.Gait analysis showed that compared with the control group,there was a significant increase of the gait linearity Δα of T.b.chinensis in the medium-and high-dose groups(all P＜0.01),and of the alternate coefficient β and the step regularity coeffi-cient σ in all three dose groups(all P＜0.01),with the high-dose group showing the most significant increase.Conclusion:Short-term high alcohol intake has a significant sedative effect on T.b.chinensis,manifested by a decreased desire for exploration,disordered gait and even ataxia.
Tupaia belangeri chinensisanimal behavioralcoholopen field testgait analysis
万方数据
维普
