Effect of Panax notoginseng total saponins compound nano-vesicles on skin wound healing and vascular formation in diabetic foot ulcer rats
Objective:To investigate the effect of Panax notoginseng total saponins(PNS)compound nano-vesi-cles on skin wound healing and vascular formation in diabetic foot ulcer(DFU)rats.Methods:Male SD rats were used to construct DFU models.Twenty-seven rats with successful DFU models were randomly divided into a DFU group,a PNS group and a PNS compound nano-vesicles(PNS-CNV)group,with 9 rats in each group.An-other 12 rats with normal blood glucose levels and skin wounds were taken as Control group.The wound healing rate of each group was compared.Serum levels of inflammatory cytokines interleukin-6(IL-6),interleukin-8(IL-8),and tumor necrosis factor-α(TNF-α)were measured using enzyme-linked immunosorbent assay(ELISA);his-topathological changes in the wound tissues were evaluated using hematoxylin and eosin(HE)staining.Microves-sel density(MVD)in the wound tissues was assessed using immunohistochemical staining;protein expression of vascular endothelial growth factor(VEGF)and neurogenic locus notch homolog protein 1(Notch1)in the wound tissues was determined using western blotting.Results:After modeling,the fasting blood glucose(FBG)levels of rats in the DFU group,PNS and PNS-CNV groups were higher than those in the Control group(P＜0.05);on the 7th and 14th day of drug intervention,compared with the DFU group,the FBG of rats in the PNS and PNS-CNV groups was significantly reduced,and the PNS-CNV group was lower than the PNS group(both P＜0.05).Compared with the Control group,rats in the DFU group showed significant decreases in wound healing rate,CD34 positive expression in the wound tissues,MVD,and VEGF protein expression,while the levels of IL-6,IL-8,TNF-α in serum and Notch1 protein expression in the wound tissues were increased(both P＜0.05).Compared with the DFU group,rats in the PNS and PNS-CNV groups exhibited increases in wound healing rate,CD34 posi-tive expression in the wound tissues,MVD,and VEGF protein expression,while the levels of IL-6,IL-8,TNF-α in serum and Notch1 protein expression in the wound tissues were decreased(all P＜0.05).Compared with the PNS group,rats in the PNS-CNV group showed increases in wound healing rate,CD34 positive expression in the wound tissues,MVD,and VEGF protein expression,while the levels of IL-6,IL-8,TNF-α in serum and Notch1 protein expression in the wound tissues were decreased(all P＜0.05).Conclusion:PNS-CNV can up-regurate the expression of VEGF in the wound tissues of DFU rats,inhibit inflammation,promote vascular formation,thus accelerating wound healing,which is better than PNS.
Panax notoginseng total saponins compound nano-vesiclesdiabetic foot ulcervascular formationwound healing
万方数据
维普
