Objective:To improve the quality standard of Yigan tablet.Methods:Isatidis Radix and Polygonum cuspidatum in Yigan tablet were identified with thin layer chromatography(TLC).The content of(R,S)-Goitrin and Emodin in Yigan tablet was determined by high performance liquid chromatography(HPLC).Results:The spots of Isatidis Radix and Polygonum cuspidatum were clear,well separated and negative without interference when identified.The peak area of(R,S)-Goitrin ranged from 0.03 μg to 0.15 μg had perfect linear relationship(r= 0.9998)with the injection volume,and the average recovery rate was 97.05%;the peak area of Emodin ranged from 0.0495 μg to 0.297 μg had perfect linear relationship(r=1.0000)with the injection volume,and the average recovery rate was 99.24%.Conclusion:The quality standard can not only effectively identify Isatidis Radix and Polygonum cuspidatum but also can effectively determine the content of(R,S)-Goitrin and Emodin.The method is simple,reliable and repeatable,and can be used for the quality control of Yigan tablet.
维普
万方数据