Expression of carboxylesterase 4A in nasopharyngeal carcinoma and its prognosis and im-mune infiltration analysis
Objective:To investigate the aberrant expression of carboxylesterase 4A(CES4A)in nasopharyngeal carcinoma(NPC)and its potential association with prognosis of patients and immune infiltration.Methods:Re-verse transcription-quantitative PCR(RT-qPCR)and immunohistochemical staining were utilized to assess the mRNA and protein expression levels of CES4A in NPC tissues.The relationship between CES4A expression lev-els and clinical pathological characteristics as well as survival time of patients was analyzed.GO,KEGG,and im-mune correlation analyses were conducted using R language.Results:Compared with normal nasopharyngeal tis-sues,CES4A mRNA and protein expression levels in NPC were significantly decreased,but there was no signifi-cant correlation with clinical pathological characteristics of NPC.Receiver operating characteristic(ROC)curves were plotted based on the GEO dataset,RT-qPCR and immunohistochemical staining data,and the area under the curve(AUC)was 0.850(P＜0.05),0.6654(P=0.0037)and 0.8098(P=0.0019),respectively.Patients with low CES4A expression exhibited poor prognosis(P＜0.001),with CES4A identified as an independent prognostic fac-tor for NPC patients(P=0.00436,HR=0.168,95%CI:=0.0492-0.5730).CES4A was positively correlated with tu-mor infiltration of B cells.Conclusion:Down-regulation of CES4A expression in NPC may serve as a reliable molecular marker for the diagnosis and poor prognosis in NPC patients.Additionally,its association with the re-duced tumor infiltration of B cells suggests that CES4A can be a potential therapeutic target for NPC.
万方数据
维普
