目的:研究比较两种基质辅助激光解吸电离飞行时间质谱仪(Autof ms 1000与MALDI Biotyper)对临床常见丝状病原真菌鉴定的准确性和效率.方法:共纳入32株丝状病原真菌标准菌株和120株临床分离菌株,按照标准操作方法分别用Au-tof ms 1000与MALDI Biotyper对相关菌株进行鉴定,对相关结果及测序分子鉴定结果进行对比分析.结果:在种水平上,Au-tof ms 1000质谱仪共检出140株丝状病原真菌,检出正确率为92.1%(140/152),其中126株分值在9.0以上,2株出现错误鉴定,误鉴定比例为1.3%(2/152);MALDI Biotyper质谱仪共正确检出98株,检出正确率为64.4%,2.0分以上的菌株47株,仅3株鉴定分值大于2.3分,7株出现误鉴定.结论:Autof ms 1000与MALDI Biotyper这两种质谱鉴定系统均能用于临床常见丝状病原真菌的快速鉴定,而Autof ms 1000质谱仪更具优势.
Abstract
Objective:To compare the accuracy and efficiency of two matrix-assisted laser desorption ionization time-of-flight mass spectrometry instruments(Autof ms 1000 and MALDI Biotyper)in identifying common clini-cal filamentous fungi.Methods:This study included 32 standard strains of filamentous fungi and 120 clinical iso-lates.The strains were identified using Autof ms 1000 and MALDI Biotyper according to standard operating pro-cedures.The results were compared with sequencing molecular identification results.Results:At the species lev-el,the Autof ms 1000 mass spectrometer detected 140 strains of filamentous fungi,with a detection accuracy rate of 92.1%(140/152).Among them,126 strains had scores above 9.0,and 2 strains were misidentified,with a mis-identification rate of 1.3%(2/152).The MALDI Biotyper mass spectrometer detected 98 strains,with a detection accuracy rate of 64.4%.There were 47 strains with scores above 2.0,only 3 strains had scores above 2.3,and 7 strains were misidentified.Conclusion:Both the Autof ms 1000 and MALDI Biotyper mass spectrometry identi-fication systems can be used for rapid identification of common filamentous pathogenic fungi in clinical practice,while the Autof ms 1000 mass spectrometer has more advantages.
维普
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