摘要
目的:建立同时测定满山红疏肝颗粒中夏佛塔苷、田基黄苷、异槲皮苷、槲皮苷和原儿茶酸5种成分的含量的方法.方法:研究采用Agilent Zorbax SB-Aq-C18色谱柱(250 mm×4.6 mm,5 μm),流动相为乙腈-0.1%磷酸,梯度洗脱,体积流量0.8 mL/min,柱温30℃,检测波长为255 nm.结果:夏佛塔苷、田基黄苷、异槲皮苷、槲皮苷和原儿茶酸分别在浓度为0.097 6～1.171 2 μg/mL、0.083 3～0.999 6 μg/mL、0.046 9～0.562 8 μg/mL、0.037 4～0.448 8 μg/mL、0.011 0～0.132 0 μg/mL范围内线性关系良好,有效成分在24h内稳定性良好,重现性好,平均加样回收率分别为100.8%、100.8%,101.7%、101.3%、101.1%,RSD分别为1.1%、1.0%、0.7%、1.1%、1.3%.6批满山红疏肝颗粒中夏佛塔苷的含量在0.886 9～0.903 1 mg/g之间,田基黄苷的含量在0.191 7～0.203 4 mg/g之间,异槲皮苷的含量在0.082 7～0.105 2 mg/g之间,槲皮苷的含量在0.009 8～0.014 0 mg/g之间,原儿茶酸的含量在0.065 5～0.069 4 mg/g之间.结论:所建立的方法简便准确、专属性强、重现性好,为满山红疏肝颗粒建立了有效可行的质量控制体系.
Abstract
Objective:To establish a method for the simultaneous determination of the contents of five compo-nents:schaftoside,vincetoxicoside B,isoquercitrin,quercitrin and protocatechuic acid in Manshanhong Shugan granules.Methods:The study employed an Agilent Zorbax SB-Aq-C18 chromatographic column(250 mm×4.6 mm,5 μm),with acetonitrile-0.1%phosphoric acid as the mobile phase,employing gradient elution at a volume flow rate of 0.8 mL/min,a column temperature of 30℃,and detection wavelength of 255 nm.Results:Schafto-side,vincetoxicoside B,isoquercitrin,quercitrin and protocatechuic acid exhibited good linear relationships with-in the concentration ranges of 0.0976 to 1.1712 μg/mL,0.0833 to 0.9996 μg/mL,0.0469 to 0.5628 μg/mL,0.0374 to 0.4488 μg/mL,and 0.0110 to 0.1320 μg/mL,respectively.The active ingredients showed good stability within 24 hours,with good reproducibility.The average sample recovery rates were 100.8%,100.8%,101.7%,101.3%,and 101.1%,respectively.The relative standard deviations(RSD)were 1.1%,1.0%,0.7%,1.1%,and 1.3%,re-spectively.In six batches of Manshanhong Shugan granules,the content of schaftoside ranged from 0.8869 to 0.9031 mg/g,vincetoxicoside B ranged from 0.1917 to 0.2034 mg/g,isoquercitrin ranged from 0.0827 to 0.1052 mg/g,quercitrin ranged from 0.0098 to 0.0140 mg/g,and protocatechuic acid ranged from 0.0655 to 0.0694 mg/g.Conclusion:The established method is simple,accurate,specific and reproducible,establishing an effective and feasible quality control system for Manshanhong Shugan granules.
基金项目
南宁市科学研究与技术开发计划科技重大专项(20213010)
维普
万方数据