Tributyrin alleviating 2,4,6-trinitrobenzene sulfonic acid-induced intestinal inflammation in rats by inhibiting ferroptosis
Objective:To investigate the mechanism of tributyrin(TB)in alleviating 2,4,6-trinitrobenzene sulfon-ic acid(TNBS)-induced intestinal inflammation in rats with experimental colitis.Methods:A total of 24 male Sprague Dawley rats were randomly divided into four groups(6 rats in each group):control group,model group(TNBS group),low-dose TB group(TNBS+TB 600 mg/kg)and high-dose TB group(TNBS+TB 1,000 mg/kg),and the low-dose and high-dose TB groups were gavaged with the corresponding dose of TB for one week before and after modeling.After modeling,the rats were observed for activity and fecal characteristics,the rats were as-sessed for the disease activity index(DAI),and the colonic tissues were observed and stained with hematoxylin-eosin and scored for histological damage after the rats were put to death.Enzyme-linked immunosorbent assay(ELISA),western blotting,immunohistochemistry,and biochemical assays were used to assess the inflammation and the results of signaling pathways associated with ferroptosis.Results:Compared with the control group,the TNBS group had significantly higher DAI scores,histological damage scores,significantly lower protein expres-sion levels of GPX4 and SLC7A11,and higher expression levels of ACSL4 and FTH1.Low-and high-dose TB treatment significantly reduced DAI scores and histological damage scores compared with the TNBS group,with the best treatment effect in the high-dose TB group(all P＜0.05).TB reversed the expression of glutathione and malondialdehyde in intestinal epithelial cells from the TNBS group and up-regulated the GPX4 and SLC7A11 protein expression levels while down-regulating the ACSL4 and FTH1 expression levels(all P＜0.05).Conclu-sion:TB ameliorates oxidative stress and intestinal inflammatory damage in TNBS-induced Crohn's disease rats.Its mechanism may be related to the inhibition of the ferroptosis pathway.
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维普
