首页|外阴阴道念珠菌病分离光滑念珠菌分子流行特征分析

外阴阴道念珠菌病分离光滑念珠菌分子流行特征分析

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
目的:调查苏州地区外阴阴道念珠菌病(VVC)分离光滑念珠菌种群分子流行特征,为VVC的防治提供流行病学依据.方法:收集2021年1-12月在苏州高新区人民医院就诊的VVC患者分离的132株光滑念珠菌,6个管家基因联合的MLST方法进行序列分型(STs),遗传分化指数(Fst)、Tajima's D和Fu's&Li's Fs中性检验用于种群遗传多样性分析.结果:132株光滑念珠菌经MLST鉴定37种STs,ST7为主,占47.0%(62/132株),而56.8%菌株拥有单一序列型(21/37 STs);经NCBI光滑念珠菌序列型数据库比对,67.6%(25/37 STs)序列型为首次出现,即新定义序列型,其中76%(19/25 STs)为单一序列型;ST7在不同临床类型VVC分离光滑念珠菌中均为主要序列型,VVC、复发性VVC(RVVC)和定植分离光滑念珠菌3个种群间遗传多样性分析显示:STs分析遗传差异小,但管家基因UGP1在致病种群(VVC/RVVC)与定植种群间遗传差异为中度(Fst:0.054 83～0.113 14).结论:苏州地区VVC患者分离光滑念珠菌种群呈高度遗传多样性和明显的地域特征,提示苏州地区特殊环境的压力选择,并为临床VVC精准防治提供流行病学依据.
Molecular epidemiological characteristics of Candida glabrata isolated from vulvovaginal can-didiasis
Objective:To investigate the molecular epidemiological characteristics of Candida glabrata(C.gla-brata)population isolated from vulvovaginal candidiasis(VVC)in Suzhou,and to provide epidemiological evi-dence for the prevention and treatment of VVC.Methods:A total of 132 isolates of C.glabratawere collected from patients diagnosed as VVC in the people's hospital of SND from January to December 2021.Multilocus se-quence typing(MLST)involving six housekeeping genes was uesd for sequence tpying(STs).Wright's fixation index(Fst),Tajima's D,and Fu's&Li's Fs neutral tests were calculated for analysis of population genetic diver-sity.Results:A total of 37 STs were identified among 132C.glabrataisolates.ST7 was dominant sequence type,accounting for 47.0%(62/132 isolates),and 56.8%(21/37 STs)were singleton sequence types.According to NC-BI sequence type database,67.6%(25/37 STs)of the sequences appeared for the first time,namely newly defined sequence types,76%(19/25 STs)of which were singleton sequence types.ST7 was the main sequence type in dif-ferent clinical types of C.glabrataisolated from VVC.The genetic diversity among 3 populations of VVC,RV-VC,and colonized C.glabratashowed that the genetic difference of the housekeeping gene UGP1 between MLST STs analysis was small,but the genetic difference between the pathogenic population(VVC/RVVC)and the colonized population was moderate(Fst:0.05483-0.11314).Conclusion:The C.glabratapopulation isolated from patients with VVC in Suzhou exhibits high genetic diversity and obvious regional characteristics,indicating the special environmental pressure selection in Suzhou,and providing epidemiological evidence for the precise prevention and treatment of VVC.

Candida glabratavulvovaginal candidiasismultilocus sequence typingpopulation genetic diversity

许雪、张璐、孙艳霞、胡丹阳、邓淑文、赵蓉芬

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苏州高新区人民医院医学研究中心,苏州 215129

苏州高新区人民医院检验科,苏州 215129

Life Science College, University of Essex, Colchester CO43SQ

光滑念珠菌 外阴阴道念珠菌病 多位点序列分型 种群遗传多样性

苏州市科技计划苏州市科技计划苏州高新区人民医院科学创新基金苏州高新区人民医院科学创新基金苏州高新区人民医院科学创新基金

SKY2022037SKYD2022092SYG2020A05SGY2021H01SGY2021C01

2024

10.16190/j.cnki.45-1211/r.2024.04.003

广西医科大学学报
广西医科大学

广西医科大学学报

CSTPCD
影响因子：0.788
ISSN：1005-930X
年,卷(期)：2024.41(4)
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